GO_REF:0000118

TreeGrafter-generated GO annotations

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

file:PSEPK/moaB-II/moaB-II-uniprot.txt

UniProtKB entry for moaB-II

UniProt places Q88E67 in the proteobacterial MoaB family and transfers GTP/MPT binding but no MPT adenylyltransferase activity.

"CC -!- FUNCTION: May be involved in the biosynthesis of molybdopterin. Can CC bind GTP and has low GTPase activity. Can bind MPT, but has no MPT CC adenylyl transferase activity. {ECO:0000256|ARBA:ARBA00055616}."

file:PSEPK/moaB-II/moaB-II-goa.tsv

QuickGO GOA annotations for moaB-II

The fetched table contains the cytosol and Mo-molybdopterin biosynthesis rows reviewed here.

"UniProtKB Q88E67 moaB-II located_in GO:0005829 cytosol cellular_component ECO:0007826 IEA GO_REF:0000118 PANTHER:PTN002456466 160488 Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) TreeGrafter Molybdenum cofactor biosynthesis protein B 20260706 UniProtKB Q88E67 moaB-II involved_in GO:0006777 Mo-molybdopterin cofactor biosynthetic process biological_process ECO:0000501 IEA GO_REF:0000120 InterPro:IPR008284|InterPro:IPR012245|InterPro:IPR013484|UniRule:UR000143783 160488 Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) UniProt Molybdenum cofactor biosynthesis protein B 20260706"

file:PSEPK/moaB-II/moaB-II-deep-research-openscientist.md

OpenScientist gene research for moaB-II

The report explicitly acknowledges that no target-specific experimental study was found.

"The caveat is not mis-identification but *evidence type*: there is **no primary experimental study of the P. putida protein Q88E67 itself**; its annotation is by homology to characterized family members."
The report leaves the exact physiological function of Q88E67 unresolved.

"- The exact physiological reaction (if any) uniquely catalyzed by MoaB-II. In E. coli, MoaB is dispensable; whether *P. putida* MoaB-II is redundant, conditionally required, or subfunctionalized (e.g., under specific Mo/W or substrate conditions) is untested."
The report presents target-specific pairwise alignment results that were not saved as a reproducible local analysis.

"Pairwise global alignments (Needleman–Wunsch, identity scoring) place moaB-II firmly in the **MoaB clade, not the MogA clade**:"

file:interpro/panther/PTHR43232/PTHR43232-entries.csv

PANTHER entries for the MoaB family

PTHR43232 contains MoaB proteins, including biochemically active archaeal MoaB, but does not by itself resolve each bacterial paralog.

"Q8U3T3,Molybdopterin adenylyltransferase,protein,186497,Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1),Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1),moaB,169,PTHR43232:SF2,MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN B,True"