GO_REF:0000002

Gene Ontology annotation through association of InterPro records with GO terms

GO_REF:0000003

Gene Ontology annotation based on Enzyme Commission mapping

GO_REF:0000033

Annotation inferences using phylogenetic trees

GO_REF:0000117

Electronic Gene Ontology annotations created by ARBA machine learning models

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

PMID:11181564

Chromosomal localization and genomic organization for the galactose/ N-acetylgalactosamine/N-acetylglucosamine 6-O-sulfotransferase gene family.

The genomic study separates human GST-4alpha and GST-4beta into adjacent open reading frames and describes the family as Golgi-resident glycan sulfotransferases.

PMID:11278593

Human corneal GlcNac 6-O-sulfotransferase and mouse intestinal GlcNac 6-O-sulfotransferase both produce keratan sulfate.

Human corneal GlcNAc-6 sulfotransferase transfers sulfate to GlcNAc C6 in synthetic acceptors and cooperates with the Gal-6 enzyme to produce keratan-reactive glycans in cells.

PMID:11352640

Sulfation of endothelial mucin by corneal keratan N-acetylglucosamine 6-O-sulfotransferase (GST-4beta).

Human corneal GST-4beta/CHST6 sulfates GlyCAM-1 in COS-7 coexpression; this establishes a mucin-glycan substrate capability without demonstrating an endogenous adhesion role.

PMID:17690104

Enzymes responsible for synthesis of corneal keratan sulfate glycosaminoglycans.

CGn6ST cooperates with beta3GnT7 and beta4GalT4 to synthesize extended GlcNAc-sulfated chains; subsequent Gal-6 sulfation produces highly sulfated keratan.

Reactome:R-HSA-2022854

Keratan sulfate biosynthesis

The keratan pathway separates GlcNAc sulfation, Gal sulfation and glycan extension; CHST6 participates through its sulfate-transfer step.

Reactome:R-HSA-2046222

CHST2,3,5,6 transfer sulfate to GlcNAc on keratan chain

The Golgi catalyst set explicitly resolves to full-length human CHST6 and models GlcNAc sulfation alongside other sulfotransferases.

Reactome:R-HSA-3656269

Defective CHST6 does not transfer SO4(2-) to GlcNAc residues on keratan-PG

The disease event models loss of normal CHST6 sulfate transfer by listed variant proteins; it is not a positive activity assay of those mutants.

PMID:11042394

Functional expression and genomic structure of human N-acetylglucosamine-6-O-sulfotransferase that transfers sulfate to beta-N-acetylglucosamine at the nonreducing end of an N-acetyllactosamine sequence.

The study identifies a human GlcNAc-6 sulfotransferase and its acceptor specificity, providing family context for the Reactome catalyst set.

PMID:10491328

Cloning and characterization of a mammalian N-acetylglucosamine-6-sulfotransferase that is highly restricted to intestinal tissue.

Human and mouse intestinal GlcNAc-6 sulfotransferase homologs were cloned; COS-cell expression supports GlcNAc-6 sulfation.

PMID:12218059

Enzymatic synthesis in vitro of the disulfated disaccharide unit of corneal keratan sulfate.

Human corneal enzyme and mouse intestinal enzyme accept longer poly-N-acetyllactosamine substrates, whereas the human intestinal enzyme does not; a four-enzyme reconstitution produces a keratan structural unit.

PMID:11017086

Macular corneal dystrophy type I and type II are caused by distinct mutations in a new sulphotransferase gene.

Human CHST6 coding changes or upstream rearrangements are associated with macular corneal dystrophy, and an affected type-II cornea lacks detectable CHST6 transcripts.

PMID:15013869

Novel mutations in the carbohydrate sulfotransferase gene (CHST6) in American patients with macular corneal dystrophy.

The human cohort identifies coding variants associated with macular corneal dystrophy and classifies antigenic keratan sulfate in serum.

file:human/CHST6/CHST6-source-evidence.json

CHST6 source identities, catalytic scope and review provenance

The authored audit preserves all 30 machine assertions and records source identity, ontology, actual access and source-selection scope.