DNAJC28 PN Consistency Notes
- Generated: 2026-06-18
- Project: PROTEOSTASIS
- Scope: PN consistency rereview against local AIGR review and available deep-research artifacts
- UniProt: Q9NX36
- AIGR review status: COMPLETE
- Review batch: proteostasis-batch-2026-06-07b
- Batch change status: added
Source Files Checked
Deep Research Files
- No
*-deep-research*.md file found in this gene directory.
AIGR Review Snapshot
- Description: DNAJC28 (C21orf55/C21orf78) is a poorly characterized member of the DnaJ/HSP40 (J-domain) co-chaperone family encoded on chromosome 21. It contains a canonical J domain together with a predicted coiled-coil region, and its N-terminus resembles a mitochondrial-targeting presequence, suggesting it may act as a mitochondrial J-domain co-chaperone. By analogy to other J-domain proteins it is presumed to assist HSP70-type chaperones in protein folding, but no substrate, partner chaperone, or cellular process has been experimentally established. It is expressed in brain, testis, uterus, spleen and liver (tissue-enhanced in testis) and is phosphorylated at Thr-347.
- Existing/core annotation action counts: KEEP_AS_NON_CORE: 1; MARK_AS_OVER_ANNOTATED: 1
PN Consistency Summary
- Consistency: COMPARTMENT MISMATCH. Deep research and review YAML agree DNAJC28 is an essentially uncharacterized J-domain protein whose N-terminus resembles a MITOCHONDRIAL targeting presequence (review/notes predict mitochondrial localization; review core MF GO:0051087 protein-folding chaperone binding). The PN row places it in the ER proteostasis branch, which conflicts with the review's mitochondrial prediction. Neither localization is experimentally confirmed, so this is an unresolved discrepancy rather than a proven error, but the branch assignment and the review point in different directions.
- PN story / NEW pressure: PN asserts GO:0030544 Hsp70 protein binding (verified real). GOA has only GO:0005515 protein binding (one IPI) + GO:0001659 (IBA, marked over-annotated), so "more_specific_than_existing_goa" is loose; effectively new_to_goa. GO:0030544 is a child of the review's GO:0051087, i.e. a narrower, defensible domain-level ADD — but purely predicted.
- Evidence alignment: PN row no titles; review's sole literature ref is PMID:24407287 (PML/ASC inflammasome — source of the single IPI only, relevance LOW, VERIFIED). No experimental HSP70-binding evidence on either side.
- Verdict: Consistent on function (uncharacterized J-protein); GO:0030544 defensible inferred ADD. Recommended edits: [MAP] change goa_status to new_to_goa; [MAP] reconcile ER vs mitochondrial branch placement (review predicts mitochondrial targeting).
Full Consistency Review
- UniProt: Q9NX36 (C21orf55/C21orf78) · batch: proteostasis-batch-2026-06-07b · review status: COMPLETE
- PN placement:
ER proteostasis|Chaperone|HSP70 system|J-domain containing HSP70 cochaperone ; PN-node mapping: type=mapped, scope=ok_for_propagation_to_go, GO:0030544 Hsp70 protein binding (projected more_specific_than_existing_goa); group/class/branch=no_mapping.
- Consistency: COMPARTMENT MISMATCH. Deep research and review YAML agree DNAJC28 is an essentially uncharacterized J-domain protein whose N-terminus resembles a MITOCHONDRIAL targeting presequence (review/notes predict mitochondrial localization; review core MF GO:0051087 protein-folding chaperone binding). The PN row places it in the ER proteostasis branch, which conflicts with the review's mitochondrial prediction. Neither localization is experimentally confirmed, so this is an unresolved discrepancy rather than a proven error, but the branch assignment and the review point in different directions.
- PN story / NEW pressure: PN asserts GO:0030544 Hsp70 protein binding (verified real). GOA has only GO:0005515 protein binding (one IPI) + GO:0001659 (IBA, marked over-annotated), so "more_specific_than_existing_goa" is loose; effectively new_to_goa. GO:0030544 is a child of the review's GO:0051087, i.e. a narrower, defensible domain-level ADD — but purely predicted.
- Mapping strategy: Node status unchanged. Two issues: (1) goa_status should be new_to_goa; (2) the ER-branch placement is questionable given the mitochondrial-presequence prediction — flag for reconciliation. Term direction (narrower than review) is appropriate.
- Evidence alignment: PN row no titles; review's sole literature ref is PMID:24407287 (PML/ASC inflammasome — source of the single IPI only, relevance LOW, VERIFIED). No experimental HSP70-binding evidence on either side.
- Verdict: Consistent on function (uncharacterized J-protein); GO:0030544 defensible inferred ADD. Recommended edits: [MAP] change goa_status to new_to_goa; [MAP] reconcile ER vs mitochondrial branch placement (review predicts mitochondrial targeting).
PN Dossier Context
- review_batch: proteostasis-batch-2026-06-07b
- review_yaml: genes/human/DNAJC28/DNAJC28-ai-review.yaml
- PN workbook rows: 1
PN row 1: ER proteostasis | Chaperone | HSP70 system | J-domain containing HSP70 cochaperone
- UniProt: Q9NX36
- In branches: ER
- PN-node mapping records (path + ancestors):
- [type] ER proteostasis|Chaperone|HSP70 system|J-domain containing HSP70 cochaperone
status=mapped scope=ok_for_propagation_to_go GO=[GO:0030544 Hsp70 protein binding]
rationale: In the PN hierarchy, this type denotes J-domain cochaperones assigned to the HSP70 system. Their shared mechanistic role is direct interaction with HSP70-family chaperones, making Hsp70 protein binding the most defensible GO target in the current cache.
- [group] ER proteostasis|Chaperone|HSP70 system
status=no_mapping scope= GO=[]
rationale: Reviewed as a broad PN category rather than a single GO class. The member genes span multiple activities, complexes, or contexts, so direct propagation from this node would overstate the shared biology.
- [class] ER proteostasis|Chaperone
status=no_mapping scope= GO=[]
rationale: Reviewed as a broad PN category rather than a single GO class. The member genes span multiple activities, complexes, or contexts, so direct propagation from this node would overstate the shared biology.
- [branch] ER proteostasis
status=no_mapping scope= GO=[]
rationale: Reviewed as a top-level PN branch. This is a systems/taxonomy umbrella, not a direct GO assertion; narrower child curations carry any propagating GO mappings.
Projected GO annotations (1)
- GO:0030544 Hsp70 protein binding | scope=ok_for_propagation_to_go | goa_status=more_specific_than_existing_goa | from=ER proteostasis|Chaperone|HSP70 system|J-domain containing HSP70 cochaperone
Note
This file is generated from the current PROTEOSTASIS phase-1 dossier and local gene-review artifacts. Edit the source review, PN mapping, or dossier rather than this generated note when correcting the underlying curation.