Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
A novel mitochondrial outer membrane protein, MOMA-1, that affects cristae morphology in Caenorhabditis elegans.
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YFP::FIS-1 is used as a reference marker for a bona fide mitochondrial outer membrane protein in C. elegans muscle cells, supporting mitochondrial outer membrane localization of FIS-1.
"Fluorescence labeling showed a pattern similar to patterns observed with YFP fused to a bona fide mitochondrial OM protein (YFP::FIS-1)"
Mutations in Fis1 disrupt orderly disposal of defective mitochondria.
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C. elegans fis-1/fis-2 single and double mutants have wild-type mitochondrial morphology; Fis1 homologs have no obvious effect on mitochondrial or peroxisomal fission, unlike Mff/Drp1.
"Our results show that fis-1 and fis-2 single and double mutants have wild-type mitochondrial morphologies, as also shown by others"
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Loss of fis-1/fis-2 causes accumulation of large LGG-1 (LC3) autophagic aggregates containing mitochondrial remnants and DRP-1; FIS-1 acts downstream of DRP-1/MFF fission to guide mitophagic disposal.
"causing the formation of large aggregates containing LGG-1, DRP-1, and remnants of mitochondria"
Effects of mutations in mitochondrial dynamics-related genes on the mitochondrial response to ultraviolet C radiation in developing Caenorhabditis elegans.
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RNAi knockdown of fis-1 blocks removal of UV-C-induced mitochondrial DNA damage, similarly to drp-1, whereas fis-1 knockout alone does not affect mitochondrial morphology or development.
"RNAi knockdown of fis-1 inhibited mtDNA damage removal similarly to drp-1"
Caenorhabditis elegans drp-1 and fis-2 regulate distinct cell-death execution pathways downstream of ced-3 and independent of ced-9.
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In C. elegans, minor pro-apoptotic roles for drp-1 and fis-2 (a FIS1 homolog) are revealed in sensitized backgrounds, acting downstream of the CED-3 caspase to promote elimination of mitochondria in dying cells.
"minor proapoptotic roles for drp-1 and fis-2, a homolog of human Fis1, are revealed in sensitized genetic backgrounds"