lhfpl5a notes (Danio rerio, LHFPL tetraspan subfamily member 5a; UniProt F1Q837)

2026-09-28 — session log (DANRE_DUPLICATION batch 3, random TGD_tree sample)

Deep research: not available (Edison/Falcon 402 Payment Required; OpenAI key invalid). Not attempted, per
instructions. Literature searched by hand (Europe PMC (lhfpl5a OR lhfpl5b OR "lhfpl5" OR tmhs) AND zebrafish).
Shared pair material also in ../lhfpl5b/lhfpl5b-notes.md; pair analysis in
lhfpl5a-bioinformatics/RESULTS.md.

Accession F1Q837 (TrEMBL, 219 aa), ZFIN:ZDB-GENE-110131-8, Ensembl ENSDARG00000045023, chr11. Mutant:
astronaut (asn), allele tm290d, ENU nonsense K80X.

Origin

• Phylogeny with gar: PMID:32009898
• Both kept in 13 teleost orders: PMID:32009898
• Singh & Isambert ohnolog call cited: PMID:32009898

Protein

• PMID:32009898
• GFP-Lhfpl5a at stereocilia tips; functional (rescues tm290d): PMID:32009898
• Localization depends on Pcdh15a, Cdh23, Myo7aa: PMID:32009898
• Needed for Pcdh15a transport: PMID:28219986
• Not needed for Tmc1/Tmc2b bundle targeting (differs from mouse): PMID:32009898

Expression

• PMID:32009898
• Adult: PMID:39484049
• Ear gene repressed by prdm1a in lateral line: PMID:40825768
• ZFIN/Bgee (expression_compare.py): lhfpl5a records are all inner-ear (maculae, from Prim-5); Bgee adds bulk testis/larva.

Function

• PMID:32009898
• Lateral line unaffected in lhfpl5a mutants (FM 1-43 labeling same as WT; hair-cell numbers normal).
• Original screen misread as downstream of transduction because lateral-line microphonics were normal
  PMID:9491988

Annotation decisions

• GO:0061512 protein localization to cilium (IMP) → MODIFY to GO:0072659 protein localization to plasma membrane
  (stereocilia are not cilia; no stereocilium-specific protein localization term exists).
• GO:0060122 inner ear receptor cell stereocilium organization → KEEP_AS_NON_CORE (splaying follows loss of Pcdh15a).
• NEW: stereocilium tip (IDA, PMID:32009898).