GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

file:PSEPK/soxG/soxG-uniprot.txt

UniProtKB entry for soxG (Q88R08)

Q88R08 carries the SoxG family signatures (IPR006280, IPR007375, TIGR01375) and a folate-binding-domain superfamily match.

PMID:15946648

Crystal structure of heterotetrameric sarcosine oxidase from Corynebacterium sp. U-96.

The characterised gamma subunit contacts the alpha subunit and may have a folate-binding structure.

"The gamma subunit is in contact with two domains of alpha subunit and has possibly a folate-binding structure."

PMID:35266867

Creatine utilization as a sole nitrogen source in Pseudomonas putida KT2440 is transcriptionally regulated by CahR.

KT2440 uses tetrameric SoxBDAG to convert sarcosine to glycine.

"sarcosine is then converted to glycine and formaldehyde by the tetrameric sarcosine oxidase (SoxBDAG)"

PMID:9185627

Folate utilization by monomeric versus heterotetrameric sarcosine oxidases.

Heterotetrameric sarcosine oxidases use tetrahydrofolate as substrate.

"The results show that only the heterotetrameric sarcosine oxidases can use tetrahydrofolates as substrates"

PMID:16934831

Identification of a stable flavin-thiolate adduct in heterotetrameric sarcosine oxidase.

The heterotetramer transfers the oxidised one-carbon fragment to tetrahydrofolate and releases formaldehyde only in folate absence.

"In the absence of H 4 folate, turnover occurs at a somewhat slower rate and the sarcosine imine is hydrolyzed to glycine and formaldehyde."

PMID:15914915

Corynebacterium sp. U-96 contains a cluster of genes of enzymes for the catabolism of sarcosine to pyruvate.

The orthologous sarcosine oxidase is functionally expressed in E. coli as a soluble enzyme.

"Functional expression of sarcosine oxidase in Escherichia coli was accomplished, but the beta subunit and the betadelta complex were expressed at a low level as a soluble protein."