Gene Ontology annotation through association of InterPro records with GO terms.
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara.
Automatic assignment of GO terms using logical inference, based on inter-ontology links.
Combined Automated Annotation using Multiple IEA Methods.
At physiological expression levels the Kidd blood group/urea transporter protein is not a water channel.
-
At physiological expression levels, SLC14A1/HUT11A transports urea but NOT water. Water permeability only observed at unphysiological overexpression levels.
-
Identified HUT11A as the true physiological variant (389 aa vs 391 aa for original HUT11 clone).
-
Phloretin strongly inhibits urea transport at physiological levels.
Crystal structure of a bacterial homologue of the kidney urea transporter.
-
Urea transporters form homotrimers with each subunit containing a continuous membrane-spanning pore.
-
Establishes that urea transporters operate by a channel-like mechanism, not a carrier mechanism.
-
Selectivity filter can accommodate dehydrated urea molecules in single file.
Kidd blood group and urea transport function of human erythrocytes are carried by the same protein.
-
SLC14A1/HUT11 is the molecular basis of the Kidd (JK) blood group system.
-
Jk(a-b-) red cells lack UT-B and have defective urea transport but normal water permeability.
-
Gene maps to chromosome 18q12-q21.
Cloning and functional expression of a urea transporter from human bone marrow cells.
-
First cloning of human erythrocyte urea transporter (HUT11).
-
Encodes 43 kDa protein with 63% identity to rabbit UT2.
-
Mediates facilitated urea transport inhibited by phloretin and pCMBS.
-
No water transport observed in oocyte expression system.
Functional differentiation of the human red blood cell and kidney urea transporters.
-
Both HUT11 and HUT2 transport urea but not water.
-
Distinct pharmacological profiles: HUT11 more sensitive to phloretin and pCMBS than HUT2.
-
Thiourea is a substrate for HUT11 (Km 40 mM) but not HUT2.
HUT2 and HUT11 mediate urea transport in kidney and erythrocytes respectively
SLC-mediated transport of organic cations
Deep research report on SLC14A1
-
Recent (2023-2024) human UT-B structures resolved by cryo-EM confirm homotrimeric architecture.
-
Conserved urea recognition motif and H-bond transfer path identified.
-
Selective inhibitors characterized with distinct binding modes.