Role of the Escherichia coli glgX gene in glycogen metabolism.
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Defined deletions in glgX showed that GlgX encodes a glycogen debranching enzyme that preferentially hydrolyzes short DP3-DP4 alpha-1,6 branches and functions mainly in glycogen catabolism.
"GlgX protein is predominantly involved in glycogen catabolism by selectively debranching the polysaccharide outer chains"
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E. coli GlgX preferentially hydrolyzes chains of three or four glucose residues.
"debranching enzyme with high specificity for hydrolysis of chains consisting of three or four glucose residues"
Glycogen synthesis and mobilization module
Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Combined Automated Annotation using Multiple IEA Methods
Over 1000 genes are involved in the DNA damage response of Escherichia coli.
Purification and properties of a debranching enzyme from Escherichia coli.
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A purified E. coli debranching activity hydrolyzed alpha-1,6 linkages in phosphorylase and beta-amylase limit dextrins.
"The enzyme hydrolyzed 1,6-alpha-glucosidic linkages in phosphorylase and beta-amylase limit dextrins"
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The 1976 enzyme preparation was reported to have very low activity on glycogen and therefore to differ from other bacterial isoamylases.
"differs from the bacterial isoamylases described in other studies because of its inability to hydrolyze glycogen"
Coordinate genetic regulation of glycogen catabolism and biosynthesis in Escherichia coli via the CsrA gene product.