Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on Enzyme Commission mapping
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
The response of Caenorhabditis elegans to hydrogen sulfide and hydrogen cyanide.
-
A forward screen for H2S-sensitive mutants identified F02H6.5 (sqrd-1); the tm3378 deletion phenocopies and fails to complement the screen alleles.
"The available deletion allele of sqrd-1 (tm3378) phenocopies and fails to complement these mutants"
-
ethe-1 mutants share the H2S-sensitivity, placing sqrd-1 in the sulfide-oxidation pathway.
"is sensitive to RA/H2S, which suggests that sqrd-1 confers resistance to H2S"
-
H2S and HCN induce sqrd-1 mRNA and protein in a hif-1-dependent manner.
"hif-1(ia04) mutant animals fail to increase sqrd-1 and cysl-2 mRNA levels"
Mitochondrial Sulfide Quinone Oxidoreductase Prevents Activation of the Unfolded Protein Response in Hydrogen Sulfide.
-
Without SQRD-1, H2S exposure causes eIF2alpha phosphorylation (GCN-2 and PEK-1), translational arrest and ER and mitochondrial UPR activation.
"leads to phosphorylation of eIF2α and inhibition of protein synthesis"
Rhodoquinone-dependent electron transport chain is essential for Caenorhabditis elegans survival in hydrogen sulfide environments.
-
Homology-model docking suggests SQRD-1 binds both ubiquinone and rhodoquinone. sqrd-1(tm3378) is sulfide-sensitive in liquid culture. Isoforms differ in FAD-binding domains.
"The sqrd-1 mutant strain (sqrd-1(tm3378)) is more sensitive to sulfide than the wild-type N2 strain"
GEI-8, a homologue of vertebrate nuclear receptor corepressor NCoR/SMRT, regulates gonad development and neuronal functions in Caenorhabditis elegans.
-
In a gei-8 mutant background, RNAi against sqrd-1 regions that include 21U-RNA loci gave weak gonad migration and germline accumulation phenotypes (4.5%).
"resulted in changes in gonad arm migrations and an accumulation of germline cells"
Biochemical properties of nematode O-acetylserine(thiol)lyase paralogs imply their distinct roles in hydrogen sulfide homeostasis.
Organization of the human mitochondrial hydrogen sulfide oxidation pathway.
-
Human SQR (SQOR) uses glutathione as its sulfane-sulfur acceptor, forming glutathione persulfide.
"Human SQR uses glutathione as an acceptor forming glutathione persulfide (GSSH)"
UniProtKB C6KRP5 (sqrd-1, Caenorhabditis elegans) TrEMBL record
-
Family membership: PANTHER mitochondrial SQR subfamily and InterPro sulphide quinone reductase. The function, catalytic-activity and location text in this record is ARBA and is not cited.
"PTHR10632:SF2; SULFIDE:QUINONE OXIDOREDUCTASE, MITOCHONDRIAL"
sqrd-1 manual literature review and isoform sequence comparison
-
The C6KRP5 and O62133 gene models begin 17 residues before the GxGxxG FAD motif and have no obvious mitochondrial presequence; A0A061ADY3 and C6KRP6 lack the N-terminal FAD motif.
"Neither predicted product carries an obvious N-terminal mitochondrial presequence comparable to the ~40-residue extension of human SQOR"