Saccharomyces cerevisiae Nip7p is required for efficient 60S ribosome subunit biogenesis
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NIP7 is essential for growth and required for 60S ribosomal subunit biogenesis in yeast.
"Nip7-1p-depleted cells showed defective pre-rRNA processing, including accumulation of the 35S rRNA precursor, presence of a 23S aberrant precursor, decreased 20S pre-rRNA levels, and accumulation of 27S pre-rRNA"
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Nip7p cosediments with free 60S subunits and localizes to the nucleolus.
"A fraction of Nip7p cosedimented through sucrose gradients with free 60S ribosomal subunits but not with 80S monosomes or polysomal ribosomes, indicating that it is not a ribosomal protein"
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Nip7p is a nucleolar protein.
"in vivo localization of a Nip7p-green fluorescent protein fusion protein revealed that a significant amount of Nip7p is present inside the nucleus, most probably in the nucleolus"
Nip7p interacts with Nop8p, an essential nucleolar protein required for 60S ribosome biogenesis, and the exosome subunit Rrp43p
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Nip7p interacts with nucleolar protein Nop8p and exosome subunit Rrp43p.
"Rrp43p and a second essential protein, Nop8p, were identified in a two-hybrid screen as Nip7p-interacting proteins"
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The three proteins form a dynamic complex in 60S biogenesis.
"Biochemical evidence for an interaction was provided by the copurification on immunoglobulin G-Sepharose of Nip7p with protein A-tagged Rrp43p and Nop8p"
Structural insights into the interaction of the Nip7 PUA domain with polyuridine RNA
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The PUA domain mediates Nip7 interaction with RNA, with conserved polyuridine preference.
"Both proteins can bind specifically to polyuridine, and RNA interaction requires specific residues of the PUA domain"
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Polyuridine binding is conserved from archaea to eukaryotes.
"the PUA domain mediates Nip7 interaction with RNA and reveals that the preference for interaction with polyuridine sequences is conserved in Archaea and eukaryotic Nip7 proteins"
The NIP7 protein is required for accurate pre-rRNA processing in human cells
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Human NIP7 is required for pre-rRNA processing and ribosome subunit biogenesis.
"downregulation of NIP7 affects pre-rRNA processing, causing an imbalance of the 40S/60S subunit ratio"
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NIP7 localizes to the nuclear compartment and associates with pre-ribosomal particles.
"The NIP7 protein is restricted to the nuclear compartment and co-sediments with complexes with molecular masses in the range of 40S-80S"
The human nucleolar protein FTSJ3 associates with NIP7 and functions in pre-rRNA processing
Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Sequence and analysis of chromosome 2 of Dictyostelium discoideum
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Genomic sequence of D. discoideum chromosome 2 where nip7 resides.
"the chromosome codes for 2,799 predicted protein coding genes and 73 transfer RNA genes"
The genome of the social amoeba Dictyostelium discoideum
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Complete genome of D. discoideum encoding approximately 12,500 predicted proteins.
"chromosomes of this organism encode approximately 12,500 predicted proteins"
BioReason-Pro SFT prediction for nip7 (D. discoideum)