PANTHER family review PTHR14950: IBA propagation assessment for dcr1
Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Regulation of heterochromatic silencing and histone H3 lysine-9 methylation by RNAi.
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Deletion of the dicer (dcr1) homolog causes accumulation of complementary transcripts from centromeric repeats, loss of H3K9 methylation, and impaired centromere function, establishing RNAi-directed heterochromatin formation.
"This is accompanied by transcriptional de-repression of transgenes integrated at the centromere, loss of histone H3 lysine-9 methylation, and impairment of centromere function"
Dicer is required for chromosome segregation and gene silencing in fission yeast cells.
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Dcr1 cleaves dsRNA into ~23 nt fragments in vitro and is required for centromeric silencing and chromosome segregation.
"Deletion of the Dicer (dcr1+) gene caused slow growth, sensitivity to thiabendazole, lagging chromosomes during anaphase, and abrogated silencing of centromeric repeats"
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Dcr1 contains an N-terminal helicase/ATP-binding domain and tandem RNase III domains.
"an N-terminal helicase domain containing an ATP-binding site motif 32 M RTGAGKT 39 and a 145 DECH 148 box, and tandem C-terminal ribonuclease (RNase) III signatures"
RNA-dependent RNA polymerase is an essential component of a self-enforcing loop coupling heterochromatin assembly to siRNA production.
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Dicer, Argonaute and Rdp1 are required for heterochromatin assembly at centromeric repeats and the silent mating-type region.
"factors involved in the RNA interference (RNAi) pathway including Argonaute, Dicer, and RNA-dependent RNA polymerase are required for heterochromatin assembly at centromeric repeats and the silent mating-type region"
The nucleation and maintenance of heterochromatin by a histone deacetylase in fission yeast.
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RNAi-directed heterochromatin nucleation operates at the silent mating-type region and centromeres in parallel with the Clr3 HDAC pathway.
"acts in a distinct pathway parallel to RNAi-directed heterochromatin nucleation to recruit Clr4 and mediate H3K9 methylation at the silent mating-type region and centromeres"
ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
RNAi-dependent and -independent RNA turnover mechanisms contribute to heterochromatic gene silencing.
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RNAi is required for heterochromatin-dependent silencing of centromeric repeat transgenes and contributes at the mating-type locus.
"the RNAi pathway is required for heterochromatin-dependent silencing of transgene insertions at centromeric repeats and acts together with other pathways to silence transgenes at the silent mating-type locus"
Involvement of Dcr1 in post-transcriptional regulation of gene expression in Schizosaccharomyces pombe.
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Dcr1 contributes to post-transcriptional regulation of cellular proteins, including glycolytic enzymes.
"compatible with the existence of a post-transcriptional gene regulatory mechanism involving Dcr1 or a Dcr1 complex"
Dicer associates with chromatin to repress genome activity in Schizosaccharomyces pombe.
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Dcr1 physically associates with chromatin (euchromatic genes, ncRNA genes, retrotransposon LTRs); association is sufficient to trigger co-transcriptional silencing.
"Physical association of RNAi with chromatin is sufficient to trigger a silencing response but not to assemble heterochromatin"
An extended dsRBD with a novel zinc-binding motif mediates nuclear retention of fission yeast Dicer.
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The C-terminal extended dsRBD coordinates one zinc ion via a novel CHCC motif; zinc coordination is required for nuclear retention and heterochromatin assembly.
"coordination of zinc by the C-terminus of Dcr1 is required for its nuclear retention and thus RNAi-mediated heterochromatin assembly"
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The dsRBD binds A-form dsRNA strongly and B-form dsDNA, but this nucleic-acid binding is dispensable for Dcr1 function.
"dsRNA binding through the dsRBD is also dispensable for heterochromatin silencing and siRNA generation"
RNAi keeps Atf1-bound stress response genes in check at nuclear pores.
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RNAi components, including Dcr1, interact with chromatin at nuclear pores to repress stress-response genes via CTGS; heat stress deports Dicer to the cytoplasm.
"RNAi components interact with chromatin at nuclear pores to keep stress response genes in check"
A novel RNAi protein, Dsh1, assembles RNAi machinery on chromatin to amplify heterochromatic siRNA.
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Dcr1, with RDRC and the factor Dsh1, amplifies pericentromeric siRNA on chromatin, essential for stable heterochromatin formation.
"siRNA is amplified by the RNA-dependent RNA polymerase complex (RDRC) and the endoribonuclease Dcr1, which synthesizes dsRNA and processes the dsRNA, respectively. The amplification is essential for stable heterochromatin formation"
Dicer promotes transcription termination at sites of replication stress to maintain genome stability.
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Dcr1 specifically promotes release of RNA Pol II (and Pol I/III antisense transcription at rDNA/tDNA) at sites of replication stress, independently of other RNAi components, maintaining genome stability.
"Dcr1, but not other components of the canonical RNAi pathway, promotes the release of Pol II from the 3? end of highly transcribed genes"
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This termination role does not require siRNA biogenesis or other RNAi pathway components.
"transcriptional termination was not dependent on sRNA biogenesis or other RNAi pathway components, demonstrating for the first time a Dcr1-specific role in Pol II release"
Dicer and Hsp104 function in a negative feedback loop to confer robustness to environmental stress.
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Dicer and Hsp104 form a negative feedback loop buffering environmentally induced phenotypic variation; without Hsp104, Dicer accumulates in cytoplasmic inclusions and heterochromatin becomes unstable.
"In the absence of Hsp104, Dicer accumulates in cytoplasmic inclusions and heterochromatin becomes unstable at elevated temperatures"
RNA interference is essential for cellular quiescence.
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RNAi (Dcr1) is essential for fission yeast quiescence; dcr1Δ cells lose viability at G0 entry and cannot maintain long-term quiescence.
"RNAi mutants lose viability at G0 entry and are unable to maintain long-term quiescence"