Shared GAPDH family evidence (TDH1/TDH2/TDH3 are paralogous NAD+-dependent phosphorylating GAPDHs, EC 1.2.1.12):
- Activity partition among paralogs PMID:3905788; all three active PMID:3905788.
- Same relative expression on glucose and ethanol (supports gluconeogenic as well as glycolytic role) PMID:3905788.
- Tdh2/Tdh3 homotetramers; Tdh3 lower Vmax PMID:3905788.
- Cell wall + cytosol PMID:11158358; PMID:11158358.
- Side activity: NAD(P)H hydratase [UniProt:P00358 "FUNCTION: As a side activity, catalyzes the hydration of the"]; NADP binding IEA from IPR006424 judged over-annotation for an NAD-specific GAPDH.
- Peripheral mitochondrial pool PMID:16962558; minor lipid-particle co-fractionation PMID:10515935.
- Melatonin affinity pull-down hit PMID:31708896; weak affinity-capture evidence marked over-annotated.
- NO/S-nitrosation and apoptosis (TDH2/TDH3 IMP) PMID:17726063 (abstract only; kept non-core).
TDH2-specific:
- Chromatin association by HyCCAPP/ChIP, condition-independent PMID:27184763 -> promoter-specific chromatin binding MARK_AS_OVER_ANNOTATED.
- Protein binding IPI with ubiquitin (UBI4) from ubiquitin-conjugate proteomics PMID:15699485 -> REMOVE (uninformative; substrate status).
Decisions: as TDH1 plus REMOVE protein binding, MARK_AS_OVER_ANNOTATED promoter-specific chromatin binding, KEEP_AS_NON_CORE nucleus, apoptotic process, ROS metabolic process.