Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
A family of diverse Cul4-Ddb1-interacting proteins includes Cdt2, which is required for S phase destruction of the replication factor Cdt1.
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Identifies 18 DDB1- and CUL4-associated factors (DCAFs), including DCAF10, that co-purify with CUL4-DDB1; WD40-containing DCAFs dock onto DDB1 via a conserved WDXR motif and act as substrate receptors.
"Here, we identify 18 Ddb1- and Cul4-associated factors (DCAFs), including 14 containing WD40 repeats. DCAFs interact with multiple surfaces on Ddb1, and the interaction of WD40-containing DCAFs with Ddb1 requires a conserved "WDXR" motif."
A reference map of the human binary protein interactome.
OTUD1 Activates Caspase-Independent and Caspase-Dependent Apoptosis by Promoting AIF Nuclear Translocation and MCL1 Degradation.
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Reports that OTUD1 deubiquitinates and stabilizes DCAF10 and recruits a CUL4A-DDB1-DCAF10 complex to promote MCL1 degradation, activating caspase-dependent apoptosis in esophageal squamous cell carcinoma; single, non-replicated study.
"OTUD1 stabilizes DDB1 and CUL4 associated factor 10 (DCAF10) and recruits the cullin 4A (CUL4A)-damage specific DNA binding protein 1 (DDB1) complex to promote myeloid cell leukemia sequence 1 (MCL1) degradation, thereby activating caspase-dependent apoptotic signaling."
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Multimodal cell maps as a foundation for structural and functional genomics.
AcM-UBE2M transfers NEDD8 to CRL4 E3 ubiquitin ligase complex
NEDD8:AcM-UBE2M binds CRL4 E3 ubiquitin ligase complex
CAND1 binds CRL4 E3 ubiquitin ligase in the nucleus
COMMDs displace CAND1 from CRL4 E3 ubiquitin ligase complex
COP9 signalosome deneddylates nuclear CRL4 E3 ubiquitin ligase complex
UniProt record for human DCAF10
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UniProt summarizes DCAF10 as a possible CUL4-DDB1 substrate receptor that interacts with DDB1.
"May function as a substrate receptor for CUL4-DDB1 E3"
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UniProt carries a Bgee sperm expression cross-reference.
"Expressed in sperm and 189 other cell types or tissues."
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UniProt carries a Reactome-derived nucleoplasm GO annotation.
"GO; GO:0005654; C:nucleoplasm; TAS:Reactome."
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UniProt lists phosphoserine sites on DCAF10.
"Phosphoserine"
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UniProt lists an omega-N-methylarginine site on DCAF10.
"Omega-N-methylarginine"
DCAF10 reviewer notes
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Reviewer notes flag DCAF10 N-terminal phosphoserine and methylarginine sites as uncharacterized.
"N-terminal disordered region (1-119), with phosphoserine sites (S53, S63, S89, S92, S349) and methylarginine R134 from large-scale proteomics; no functional studies on these PTMs"
Falcon deep research report for DCAF10
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Synthesizes recent primary literature indicating DCAF10 acts as a substrate receptor of CRL4 (CUL4A/B-DDB1-RBX1) and, per Kremer et al. 2026, functions as an N-recognin recognizing N-terminally acetylated glycine (Ac-Gly) degrons, with reconstituted CUL4A-DDB1-DCAF10 directly ubiquitinating Src-family kinases (Lyn, Fyn, Src) in vitro.
"As a substrate receptor, DCAF10 recruits specific target proteins to the E3 ligase complex, facilitating their ubiquitination and subsequent proteasomal degradation"
OpenScientist report on DCAF10 Ac-Gly N-degron specificity
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The hypothesis-level review concludes that DCAF10 is now supported as an Ac-Gly N-degron recognin, while its endogenous substrate breadth beyond Src-family kinases remains unresolved.
"A specific N-degron recognin molecular function annotation is justified for DCAF10 based on direct Ac-Gly binding, reconstituted E3 ligase activity, endogenous Lyn substrate validation with genetic approaches, and degron mutagenesis."
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The proteome-wide breadth of DCAF10 Ac-Gly recognition remains uncharacterized.
"While the mechanistic evidence for DCAF10 as an Ac-Gly N-recognin is strong, **the proteome-wide breadth of its substrate recognition has not been experimentally defined**."
CUL4A-DDB1-DCAF10 is an N-recognin for N-terminally acetylated Src kinases.
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Defines DCAF10 as the CRL4 substrate receptor (N-recognin) that recognizes an N-terminally acetylated glycine (Ac-Gly) degron via a pocket in its WD40 beta-propeller, and shows reconstituted CUL4A-DDB1-DCAF10 directly ubiquitinates Src-family kinases (Lyn, Fyn, Src) when N-myristoylation is impaired. Provides direct molecular-function and process evidence for DCAF10 as a CRL4 substrate-recognition subunit.
"Using peptide pull-downs, mass spectrometry, and AlphaFold 3 predictions, we identify DCAF10 as the E3 ligase substrate receptor for alternatively N-terminally acetylated SFKs."
Adenovirus E1A binding to DCAF10 targets proteasomal degradation of RUVBL1/2 AAA+ ATPases required for quaternary assembly of multiprotein machines, innate immunity, and responses to metabolic stress.
KRAS/ABHD17C/ALOX15B Axis Promotes Pancreatic Cancer Progression via Ferroptosis Evasion.
Expression profiling of WD40 family genes including DDB1- and CUL4- associated factor (DCAF) genes in mice and human suggests important regulatory roles in testicular development and spermatogenesis.