Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping
Gene Ontology annotation based on curation of intracellular localizations of expressed fusion proteins in living cells
Electronic Gene Ontology annotations created by ARBA machine learning models
Quantitative interaction proteomics of neurodegenerative disease proteins.
A CRISPR screen defines a signal peptide processing pathway required by flaviviruses.
Inverting the Topology of a Transmembrane Protein by Regulating the Translocation of the First Transmembrane Helix.
Architecture of the human interactome defines protein communities and disease networks.
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Structure of the human signal peptidase complex reveals the determinants for signal peptide cleavage.
Interaction of chikungunya virus glycoproteins with macrophage factors controls virion production.
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In human (THP-1-derived) macrophages, SPCS3 was identified by proteomics and functional validation as a chikungunya virus (CHIKV) E1 glycoprotein-binding host protein with anti-CHIKV (restriction-factor) activity; the positively selected CHIKV E1 residue V220 is indispensable for virion production and its mutation attenuates E1 interaction with the host restriction factors SPCS3 and eIF3k, indicating a host-virus evolutionary arms race late in the viral life cycle.
Landscape of protein-protein interactions during hepatitis C virus assembly and release.
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An affinity-purification mass-spectrometry interactome of HCV-infected cells engaged SPC components near viral envelope/assembly proteins, with SPCS3 reported in the HCV E2 accessory interactome; the authors note SPC subunits SPCS1 and SPCS3 were previously recognized as important for flavivirus particle production and HCV.
Multimodal cell maps as a foundation for structural and functional genomics.
Cleavage of the signal peptide of Preproghrelin
Signalase cleaves prM-E-NS1-NS2A
Signalase cleaves prepro-NS4B
UniProt entry P61009 (SPCS3_HUMAN), signal peptidase complex subunit 3
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Essential non-catalytic accessory subunit of the ER signal peptidase complex (SPC-A with SEC11A and SPC-C with SEC11C, each also containing SPCS1 and SPCS2); single-pass type II ER membrane protein with a large lumenal domain; essential for SPC catalytic activity by stabilizing/positioning the active center; flavivirus host factor.