Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping
Ubiquitin-like protein 5 positively regulates chaperone gene expression in the mitochondrial unfolded protein response.
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First identification of UBL-5 as positive regulator of UPR-mt
"RNAi of ubl-5, a gene encoding a ubiquitin-like protein, suppresses activation of the UPR(mt) markers hsp-60::gfp and hsp-6::gfp"
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RNAi of ubl-5 suppresses hsp-60::gfp and hsp-6::gfp reporter activation
"RNAi of ubl-5, a gene encoding a ubiquitin-like protein, suppresses activation of the UPR(mt) markers hsp-60::gfp and hsp-6::gfp by the zc32 mutation"
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ubl-5 RNAi inhibits induction of endogenous hsp-60 and hsp-6 genes
"ubl-5 (RNAi) inhibits the induction of endogenous mitochondrial chaperone encoding genes hsp-60 and hsp-6 and compromises the ability of animals to cope with mitochondrial stress"
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Mitochondrial stress promotes nuclear accumulation of GFP-tagged UBL-5
"Induction of mitochondrial stress promotes accumulation of GFP-tagged UBL-5 in nuclei of transgenic worms"
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UBL-5 effects a nuclear step in UPR-mt signaling
"UBL-5 effects a nuclear step required for mounting a response to the threat of mitochondrial protein misfolding"
ClpP mediates activation of a mitochondrial unfolded protein response in C. elegans.
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DVE-1 and UBL-5 form a complex during mitochondrial stress
"Unfolded protein stress in the mitochondria correlates with complex formation between a homeodomain-containing transcription factor DVE-1 and the small ubiquitin-like protein UBL-5"
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Both DVE-1 and UBL-5 are required for signaling UPR-mt
"DVE-1 and the small ubiquitin-like protein UBL-5, both of which are encoded by genes required for signaling the UPR(mt)"
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DVE-1 nuclear redistribution correlates with UPR-mt activation
"Activation of the UPR(mt) correlates temporally and spatially with nuclear redistribution of DVE-1"
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DVE-1 binds promoters of mitochondrial chaperone genes
"Activation of the UPR(mt) correlates temporally and spatially with nuclear redistribution of DVE-1 and with its enhanced binding to the promoters of mitochondrial chaperone genes"
The NAD(+)/Sirtuin Pathway Modulates Longevity through Activation of Mitochondrial UPR and FOXO Signaling.
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NAD+ restoration promotes longevity via UPR-mt activation
"genetic or pharmacological restoration of NAD(+) prevents age-associated metabolic decline and promotes longevity in worms"
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UPR-mt is part of the mitochondrial stress signaling pathway
"activation of stress signaling via the mitochondrial unfolded protein response (UPR(mt)) and the nuclear translocation and activation of FOXO transcription factor DAF-16"
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Links metabolic regulation to mitochondrial proteostasis
"augmenting mitochondrial stress signaling through the modulation of NAD(+) levels may be a target to improve mitochondrial function"
GOA annotation export for ubl-5
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GOA records the phylogenetic IBA source for the ubl-5 GO:0031386 annotation.
"UniProtKB P91302 ubl-5 enables GO:0031386 protein tag activity molecular_function ECO:0000318 IBA GO_REF:0000033 PANTHER:PTN000315756|SGD:S000007251 6239 Caenorhabditis elegans GO_Central Ubiquitin-like protein 5 20170228"
Deep research summary for UBL-5
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UBL-5 binds HIND-containing spliceosomal proteins SART-1 and PRP-38
"UBL-5 binds HIND-containing spliceosomal proteins SART-1 and PRP-38, co-IPs with spliceosomal proteins"
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UBL-5 co-immunoprecipitates with spliceosomal components
"co-IP/MS showed enrichment of spliceosomal proteins in UBL-5 complexes in C. elegans"
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ubl-5 mutants arrest at L3 and exhibit splicing defects
"ubl-5 null mutants arrest at L3, and ubl-5 expression is elevated at L3, aligning with a critical developmental window for its splicing function"
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UBL-5 lacks C-terminal di-Gly motif for covalent conjugation
"UBL-5/Hub1 is a ubiquitin-like protein that, unlike many UBLs, lacks the C-terminal di-Gly motif for covalent conjugation"
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UBL-5 functions via non-covalent protein-protein interactions
"Not a covalent modifier... UBL-5 does not act through E1-E2-E3 conjugation but via direct protein-protein interactions in the splicing machinery"
UniProtKB entry for UBL-5 C. elegans
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73 amino acid ubiquitin-like protein
"73 AA"
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Contains Hub1/Ubl5 domain (IPR039732)
"InterPro; IPR039732; Hub1/Ubl5"
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Cytoplasmic localization by sequence similarity
"SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}"
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Interacts with DVE-1 in stress-dependent manner
"Interacts with dve-1; the interaction occurs in a mitochondrial stress-dependent manner"
OpenScientist hypothesis report for ubl-5 GO:0031386