Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
A new type of congenital disorders of glycosylation (CDG-Ii) provides new insights into the early steps of dolichol-linked oligosaccharide biosynthesis.
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Identified human ALG2 as the gene deficient in CDG-Ii; patient fibroblasts accumulate Man1GlcNAc2-PP-dolichol and Man2GlcNAc2-PP-dolichol, and hALG2 acts as an alpha-1,3-mannosyltransferase in dolichol-linked oligosaccharide biosynthesis.
"hALG2 was shown to act as an alpha1,3-mannosyltransferase"
Defining the membrane proteome of NK cells.
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Large-scale membrane-proteome MS study of an NK-like cell line that detected ALG2 among membrane-associated proteins, supporting only a generic membrane localization.
"identified 1843 proteins with high confidence scores"
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
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Systematic yeast two-hybrid interactome of neurodegenerative disease proteins that is the source of the ALG2 IntAct protein-binding interactions (with ATXN1 and HTT); not a study of ALG2 enzymatic function.
"systematic yeast two-hybrid"
Topological and enzymatic analysis of human Alg2 mannosyltransferase reveals its role in lipid-linked oligosaccharide biosynthetic pathway.
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Human ALG2 is a bifunctional alpha-1,3/alpha-1,6-mannosyltransferase that adds the second and third mannoses to Man1GlcNAc2-PP-dolichol to form branched Man3GlcNAc2-PP-dolichol.
"Alg2 catalyzes addition of both the second and third mannose to M1Gn2-PDol to form a branched"
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ALG2 localizes to the ER membrane (co-localizes with Calnexin) and both its termini and catalytic domain face the cytoplasm; in human it uses a single membrane-binding domain.
"both the N- and C-termini of hAlg2 are oriented toward cytoplasmic side of the ER"
Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
Addition of a second mannose to the N-glycan precursor by ALG2
Defective ALG2 does not transfer a second Man to N-glycan precursor