CYCLOPS (Lotus japonicus, A9XMT3) curation notes

Date: 2026-10-02. Sources: UniProt A9XMT3, GOA (14 rows), cached publications, Falcon deep research.

Identity

CYCLOPS = IPD3 orthologue (Medicago IPD3 = Interacting Protein of DMI3). 518 aa, plant-specific, no similarity to known proteins outside the CYCLOPS family (IPR040036; PANTHER PTHR36890).
PMID:19074278

Interacts with kinase-active CCaMK; preassembled complex: PMID:19074278
DNA-binding transcriptional activator: PMID:24528861
Phospho-activation: PMID:24528861 (Ser50/Ser154 per deep research.)
Targets: NIN PMID:24528861; ERN1 PMID:28503742; RAM1 PMID:27020747; CBP1 shared element (Gong et al. 2022, from deep research only; not cached).
Homodimerization by Y2H/BiFC PMID:19074278; functional relevance unknown.

Infection (rhizobial ITs; AM outer cortex entry, arbuscules): PMID:19074278
Organogenesis: dispensable when CCaMK deregulated PMID:19074278, yet phosphomimetic CYCLOPS is sufficient PMID:24528861
Conservation: rice CYCLOPS required for AM and complements Lotus mutant PMID:19074278.

GO:0003700 -> MODIFY to GO:0001216 DNA-binding transcription activator activity (all targets activated; Pol II specificity not shown so not GO:0001228).
protein binding (CCaMK, A0AAR7) -> MODIFY to GO:0019901 protein kinase binding.
GO:0006355 -> MODIFY to GO:0045893 (already present from ERN1 paper).
Homodimerization -> KEEP_AS_NON_CORE.
nucleus, sequence-specific DNA binding, nodulation, AM association, positive regulation of transcription -> ACCEPT.
Participation test for nodulation/AM: CYCLOPS is the transcription factor that directly activates NIN/ERN1/RAM1, so it performs a step (transcriptional induction) rather than only being necessary. No NEW terms added.

No defense/immunity terms on CYCLOPS; annotations are cleanly symbiosis-specific (nodulation, AM). Consistent with ORYSJ/CCAMK, where CYCLOPS is the downstream effector of CCaMK.