Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
The atu and liu clusters are involved in the catabolic pathways for acyclic monoterpenes and leucine in Pseudomonas aeruginosa.
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The atu and liu clusters are both needed for acyclic monoterpene and leucine catabolism.
"Our results suggest that the atu and liu clusters are bifunctional operons involved in both the AMTC and leucine catabolic pathways."
Identification of genes and proteins necessary for catabolism of acyclic terpenes and leucine/isovalerate in Pseudomonas aeruginosa.
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Insertion mutants show the liu cluster is essential for leucine and isovalerate utilization; P. putida has a Liu pathway but cannot grow on acyclic terpenes.
"P. fluorescens, but not P. putida, could grow on acyclic terpenes (citronellol and citronellate), while both species were able to utilize leucine and isovalerate"
Methylcrotonyl-CoA and geranyl-CoA carboxylases are involved in leucine/isovalerate utilization (Liu) and acyclic terpene utilization (Atu), and are encoded by liuB/liuD and atuC/atuF, in Pseudomonas aeruginosa.
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The Atu pathway joins the Liu pathway at methylcrotonyl-CoA; LiuB/LiuD are the MCase subunits.
"The catabolic pathway of citronellol (Atu, acyclic terpene utilization) enters that of leucine/isovalerate (Liu, leucine and isovalerate utilization) at the level of methylcrotonyl-CoA."
Biochemical characterization of isovaleryl-CoA dehydrogenase (LiuA) of Pseudomonas aeruginosa and the importance of liu genes fora functional catabolic pathway of methyl-branched compounds.
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Insertion mutants in every liu gene fail to grow on acyclic terpenes and on leucine or isovalerate.
"Mutants with insertion in any of the liu genes were unable to utilize acyclic terpenes or leucine/isovalerate"
The Pseudomonas aeruginosa liuE gene encodes the 3-hydroxy-3-methylglutaryl coenzyme A lyase, involved in leucine and acyclic terpene catabolism.
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LiuE, downstream of LiuC, is the HMG-CoA lyase.
"These results demonstrate that the liuE gene of P. aeruginosa encodes for the HMG-CoA lyase"
UniProt entry Q9I298 (Q9I298_PSEAE)