Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic Gene Ontology annotation based on Rhea mapping
Combined Automated Annotation using Multiple IEA Methods
Dictyostelium macroautophagy mutants vary in the severity of their developmental defects.
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atg1, atg6 and atg8 are all required for macroautophagy in Dictyostelium, and atg1 mutants show reduced survival during nitrogen starvation.
"genes are required for macroautophagy in Dictyostelium"
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The atg1-1 mutant shows the most severe developmental defect, failing to aggregate on bacterial lawns and arresting as loose mounds.
"does not aggregate on bacterial lawns"
Autophagy gene disruption reveals a non-vacuolar cell death pathway in Dictyostelium.
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Disruption of atg1 impairs autophagy and abolishes vacuolization in the developmental cell death model, though a non-vacuolar death still occurs.
"led in atg1 mutant cells to impaired autophagy and to no vacuolization"
Function of the Dictyostelium discoideum Atg1 kinase during autophagy and development.
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The kinase domain and conserved C-terminal domain of DdAtg1 are essential for autophagy and development; a kinase-negative mutant is dominant-negative.
"these domains are essential for autophagy and development"
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Kinase-negative DdAtg1 colocalizes with RFP-Atg8, a marker of preautophagosomal structures and autophagosomes.
"a marker of preautophagosomal structures and autophagosomes"
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In dominant-negative Atg1 cells, autophagosomes form and accumulate but are non-functional.
"In cells expressing dominant-negative DdAtg1, autophagosomes are formed and accumulate but seem not to be functional"
From autophagic to necrotic cell death in Dictyostelium.
Lipopolysaccharide induction of autophagy is associated with enhanced bactericidal activity in Dictyostelium discoideum.
The autophagic machinery ensures nonlytic transmission of mycobacteria.
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Impairing autophagy blocks nonlytic cell-to-cell transmission of mycobacteria and compromises host membrane integrity.
"If autophagy is impaired, cell-to-cell transmission is inhibited, the host plasma membrane becomes compromised and the host cells die"
Dissecting the function of Atg1 complex in Dictyostelium autophagy reveals a connection with the pentose phosphate pathway enzyme transketolase.
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The Dictyostelium Atg1 complex core is Atg1-Atg13-Atg101, more similar to the mammalian than the yeast complex.
"The core of this assembly of proteins is formed by Atg1-Atg13-Atg101, which seems to be conserved"
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Atg1 physically interacts with transketolase (TKT) and positively regulates its activity; TKT activity increases with Atg1 overexpression and decreases in atg1-null or kinase-dead cells.
"There is an increase in activity in cells overexpressing Atg1. By contrast, TKT activity decreases in cells in which atg1 has been deleted"
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Atg1-GFP has a general cytoplasmic pattern with no specific puncta localization.
"Atg1 fused to GFP has been shown previously to have a general cytoplasmic pattern in Dictyostelium cells with no specific puncta localization"
Dictyostelium discoideum as a Novel Host System to Study the Interaction between Phagocytes and Yeasts.
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atg1-null cells show altered ability to predate yeast, implicating autophagy in the amoeba-yeast interaction.
"mutants increase (atg1-, kil1-, kil2-) or decrease (atg6-) the ability of the"
Mycobacterium marinum antagonistically induces an autophagic response while repressing the autophagic flux in a TORC1- and ESX-1-dependent manner.
AMPKα promotes basal autophagy induction in Dictyostelium discoideum.
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The AMPK-ATG1 axis is conserved in basal autophagy, shown by co-immunoprecipitation.
"Co-immunoprecipitation studies show conservation of the AMPK-ATG1"
Trafficking of adhesion and aggregation-modulating proteins during the early stages of Dictyostelium development.
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Intracellular and extracellular amounts of the adhesion protein CadA are modulated by autophagy genes atg1 and atg9.
"The intracellular and extracellular amounts of CadA are modulated by autophagy genes"
The Multifaceted Interactions of Dictyostelium Atg1 with Mitochondrial Function, Endocytosis, Growth, and Development.
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Atg1 positively regulates macropinocytosis; the null mutant has a macropinocytic defect and uptake correlates with atg1 expression.
"the atg1 null mutant displayed a macropinocytic defect"
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Atg1 increases tolerance of Dictyostelium to starvation and cAMP treatment.
"Atg1 increases the tolerance of D. discoideum to starvation and cAMP treatment"
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Altered atg1 expression yields smaller fruiting bodies and multi-tipped aggregates, and Atg1 has a peripheral role in mitochondrial signalling affecting respiration.
"knocking out atg1 caused reductions in basal mitochondrial and non-mitochondrial respiration"