Use of the ND evidence code for Gene Ontology (GO) terms
Rtt101 and Mms1 in budding yeast form a CUL4(DDB1)-like ubiquitin ligase that promotes replication through damaged DNA.
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Crt10 co-purifies with Rtt101 and Mms1 (not Cdc53) by TAP-MS; the Crt10-Rtt101 interaction requires Mms1 and the Rtt101 N-terminus, whereas Crt10 binds Mms1 without Rtt101.
"The interaction between Crt10 and Rtt101 was dependent on Mms1 ( Fig 4A )."
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Crt10 associates with Rtt101 constitutively, unlike Mms22, suggesting two distinct Rtt101 complexes.
"Crt10 interacted with Rtt101 in the absence of MMS, whereas the interaction between Mms22 and Rtt101 was stimulated after MMS treatment"
Crt10 directs the cullin-E3 ligase Rtt101 to nonfunctional 25S rRNA decay.
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Crt10 is required for 25S nonfunctional rRNA decay but not for DNA repair, and forms two distinct E3 complexes, one containing the Paf1 complex.
"We also identified two distinct Crt10-containing E3 complexes, one of which contained the Paf1 complex"
The social and structural architecture of the yeast protein interactome.
Identification and characterization of CRT10 as a novel regulator of Saccharomyces cerevisiae ribonucleotide reductase genes.
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crt10 deletion confers HU resistance, partially rescues mec1 null lethality and elevates RNR2/RNR3 expression; CRT10 acts in the CRT1 (not SML1) pathway downstream of DUN1 and is required for normal CRT1 transcript levels.
"Epistatic analysis indicates that CRT10 belongs to the CRT1 pathway but not the SML1 pathway."
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CRT10 expression is induced by DNA damage and HU in a DUN1-dependent manner.
"the expression of CRT10 itself is induced by DNA damaging agents and this induction requires DUN1"
Cul8/Rtt101 forms a variety of protein complexes that regulate DNA damage response and transcriptional silencing.
UniProtKB/Swiss-Prot entry Q08226 (CRT10_YEAST)