Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on Enzyme Commission mapping
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Loss of Pseudouridine Synthases in the RluA Family Causes Hypersensitive Nociception in Drosophila.
-
RluA-1 retains the conserved RluA-family catalytic motifs; the alignment explicitly uses Q9VKV0 together with human/mouse RPUSD2 and yeast Rib2/Pus9.
"RluA-1-PABC_Dm: Q9VKV0, RluA-2-PC_Dm: Q9VKU8, RUSD2_Hs: Q8IZ73"
-
An endogenous GFSTF exon trap localizes RluA-1 to sensory-neuron nuclei.
"a GFP exon trap for RluA-1 protein localized to the nuclei of these neurons."
-
Loss of RluA-1 causes hyperalgesia rescued by nociceptor cDNA expression, and hyperbranched class IV dendrites; the RNA target mediating these phenotypes is unknown.
"Tissue-specific RNAi, genetic null mutant, and cDNA rescue experiments all indicate that loss of the RluA-1 gene from whole animals, or specifically from nociceptors, results in hyperalgesia."
Pseudouridylation at position 32 of mitochondrial and cytoplasmic tRNAs requires two distinct enzymes in Saccharomyces cerevisiae.
-
Yeast SF40 members Rib2/Pus8 (cytoplasmic) and Pus9 (mitochondrial) are required for tRNA Psi32.
"two enzymes, Rib2/Pus8p and Pus9p, are required for Psi32 formation in cytoplasmic and mitochondrial tRNAs, respectively"
A comprehensive tRNA pseudouridine map uncovers targets dependent on human stand-alone pseudouridine synthases.
-
Human RPUSD2 (SF40) modifies the first unpaired anticodon-loop uridine of tRNAs and has lost the bacterial RluA rRNA activity.
"Compared with bacterial RluA, eukaryotic RPUSD2 has lost its activity towards rRNAs, while its function on tRNA Ψ32 is well maintained."
Quantitative analysis of small RNA pseudouridylation reveals interplay of PUS enzymes in tRNA anticodon stem-loop.
-
Human RPUSD2 pseudouridylates Psi31 and Psi32 of mitochondrial tRNAs.
"RPUSD2 pseudouridylates Ψ31 of mt-tRNALeu(CUN), Ψ32 of mt-tRNAPro and mt-tRNACys"
FlyBase gene description for RluA-1 (DROME)