A C-methyltransferase involved in both ubiquinone and menaquinone biosynthesis; isolation and identification of the Escherichia coli ubiE gene
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E. coli ubiE encodes a C-methyltransferase required for C-methylation in both ubiquinone and menaquinone biosynthesis.
"Strains of Escherichia coli with mutations in the ubiE gene are not able to catalyze the carbon methylation reaction in the biosynthesis of ubiquinone (coenzyme Q) and menaquinone (vitamin K2), essential isoprenoid quinone components of the respiratory electron transport chain"
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ubiE mutants accumulate demethylmenaquinone and 2-octaprenyl-6-methoxy-1,4-benzoquinone.
"E. coli strains containing either the disruption or the point mutation in ubiE accumulated 2-octaprenyl-6-methoxy-1,4-benzoquinone and demethylmenaquinone as predominant intermediates"
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UbiE contains SAM-binding motifs common to SAM-dependent methyltransferases.
"we evaluated the amino acid sequences encoded by open reading frames located in this region for the presence of sequence motifs common to a wide variety of S-adenosyl-L-methionine-dependent methyltransferases"
Characterization of the COQ5 gene from Saccharomyces cerevisiae. Evidence for a C-methyltransferase in ubiquinone biosynthesis
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Yeast COQ5 has 44% identity to E. coli UbiE and is required for a C-methyltransferase step in ubiquinone biosynthesis.
"with 44% sequence identity over 262 amino acids to UbiE, which is required for a C-methyltransferase step in the Q and menaquinone biosynthetic pathways in Escherichia coli. Both the ubiE and COQ5 coding sequences contain sequence motifs common to a wide variety of S-adenosyl-L-methionine-dependent methyltransferases"
Mycobacterial MenG; Partial Purification, Characterization, and Inhibition
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Mycobacterial MenG (Rv0558) is a SAM/demethylmenaquinol methyltransferase that complements E. coli ubiE deletion.
"Heterologous expression of Rv0558 complemented an ubiE (the quinone C-methyltransferase involved in ubiquinone and menaquinone synthesis) deletion in Escherichia coli, and expression in a wild-type E. coli strain increased quinone C-methyltransferase specific activity by threefold"
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The reduced form demethylmenaquinol, not the oxidized demethylmenaquinone, is the true substrate.
"addition of dithiothreitol, dithionite, NADH, or other substrates of primary dehydrogenases to reaction mixtures containing membrane preparations stimulated the activity. Thus, these observations strongly suggest that demethylmenaquinol is the actual substrate of MenG"
Identification of Escherichia coli ubiB, a gene required for the first monooxygenase step in ubiquinone biosynthesis
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ubiE is the first gene in an operon with yigP and ubiB in E. coli.
"the ubiE gene encodes a C-methyltransferase required for the synthesis of both CoQ and menaquinone, and it is the 5' gene in an operon containing ubiE, yigP, and ubiB"
Characterisation of Escherichia coli K-12 mutants defective in formate-dependent nitrite reduction; essential roles for hemN and the menFDBCE operon
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In E. coli, menaquinone (but not demethylmenaquinone) is essential for certain anaerobic electron transfer pathways.
"combined data establish that menaquinones are essential for cytochrome-c-dependent trimethylamine-N-oxide reductase (Tor) and Nrf activity, but that either menaquinone or ubiquinone, but not demethylmenaquinone, can transfer electrons to a third cytochrome-c-dependent electron transfer chain, the periplasmic nitrate reductase"
The O(2)-independent pathway of ubiquinone biosynthesis is essential for denitrification in Pseudomonas aeruginosa.
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Ubiquinone-9 is the major quinone in P. aeruginosa and is required for anaerobic denitrification.
"we established that UQ9 is the major quinone of Pseudomonas aeruginosa and is required for growth under anaerobic respiration (i.e. denitrification)"
Function of ubiquinone in the electron transport system of Pseudomonas aeruginosa grown aerobically
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Ubiquinone is indispensable for the aerobic electron transport chain of P. aeruginosa.
"Complete removal of ubiquinone performed by extracting the lyophilized membrane particles with n-pentane containing acetone resulted in complete loss of all oxidase activities for glucose, gluconate, malate, succinate, and NADH"
Membrane-bound respiratory chain of Pseudomonas aeruginosa grown aerobically
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Aerobically grown P. aeruginosa uses coenzyme Q9 in its respiratory chain.
"The electron transport chain of the gram-negative bacterium Pseudomonas aeruginosa, grown aerobically, contained a number of primary dehydrogenases and respiratory components (soluble flavin, bound flavin, coenzyme Q9, heme b, heme c, and cytochrome o)"
Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen
Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods