NCU08990 encodes eL39, a small basic structural protein of the cytosolic large ribosomal subunit. It is present in the experimentally determined Neurospora 80S ribosome and participates in cytoplasmic protein synthesis. Characterized fungal eL39 contributes to ribosome integrity and translational accuracy.
The exact current accession is Q7S2X9; NCU08990 is retained as the current locus identifier. No independently established formal gene-symbol replacement was found. Original claims are preserved in NCU08990-protnlm-source.json from the live API snapshot retrieved 2026-09-09T03:00:51.831347+00:00. The current sequence is not proven to be the prediction-time input, and placeholder API dates do not establish release or training membership.
The specific domain/family observation used is DR InterPro; IPR000077; Ribosomal_eL39.. Family membership and characterized relatives establish the inference; ARBA or AI-generated names are not independent functional evidence. PAINT is a curator-reviewed ancestral assertion, not donor-count evidence.
No unresolved GOA assertion remains at the reviewed level of specificity. Experimental observations on relatives are distinguished from target observations.
PDB 7R81 entity 74 is L39, chain VB (author chain n1), 51 residues, mapped explicitly to Q7S2X9 by RCSB. Its raw entity JSON is retained in NCU08990-7R81-entity74.json. This identifies the target protein in the Neurospora translating 80S complex despite the paper abstract foregrounding drug-binding proteins. Source: https://www.rcsb.org/structure/7R81 and https://data.rcsb.org/rest/v1/core/polymer_entity/7R81/74.
The actual Falcon report arrived after the wrapper timeout and was inspected. It supports the eL39 family role but missed the direct Neurospora translating-ribosome structure (PMID:34815343; PDB 7R81). Its statement that direct incorporation evidence was not located describes its search, not the full evidence now available: retained RCSB entity 74 explicitly maps the structure to Q7S2X9. The direct target model therefore supplies stronger support than the report's distant-family examples. No untested specialized-ribosome or chaperone-regulation role is added. The wrapper reported a 600-second timeout and fallback HTTP 401 insufficient quota, but the original child completed without duplicate submission.
Scope: re-examined all 8 GOA rows, the description and core functions; no ProtNLM2 file consulted.
Identity: Q7S2X9 is the full-length NCU08990 product (51 aa, unreviewed TrEMBL). eL39 proteins are naturally
~51 aa (yeast P04650, human P62891, S. pombe P05767 are all 51 aa), so this is not a fragment, isoform or
alternative gene model. UniProt maps it to PDB 7R81 chain n1 over residues 1-51 ("DR PDB; 7R81; EM; 2.70 A; n1=1-51.").
Evolutionary grounding (UniProt REST, checked today):
- Q7S2X9: PTHR19970:SF0 (LARGE RIBOSOMAL SUBUNIT PROTEIN EL39).
- S. cerevisiae RPL39 P04650: PTHR19970:SF0. S. pombe rpl39 P05767: PTHR19970:SF0. Human RPL39 P62891: PTHR19970:SF0.
- Human RPL39L Q96EH5: PTHR19970:SF22 (different subfamily). The PAINT spermatogenesis IBD (GO:0007283) sits on
eutherian node PTN004522638 seeded by RPL39L orthologs and is correctly not propagated to fungi.
- Root node PTN000463054 carries GO:0022625, GO:0003735 and GO:0002181 (fungal, plant and mammalian seeds).
Decisions: IBA/IEA structural constituent of ribosome, cytosolic large ribosomal subunit and cytoplasmic
translation ACCEPT; IEA ribosome -> MODIFY to GO:0022625; IEA translation -> MODIFY to GO:0002181; IEA
preribosome changed from ACCEPT to KEEP_AS_NON_CORE (transient assembly state, ortholog transfer only).
Supporting evidence for target-level claims now cites the UniProt 7R81 chain mapping rather than relying only on
the CHX-focused abstract. Description rewritten as a standalone summary.
Family review interpro/panther/PTHR19970/PTHR19970-review.yaml agrees with SF0 placement; its notes say no
accession-matched structural chain is used, but UniProt does map Q7S2X9 to 7R81 chain n1 (no placement conflict).
Closest characterised relative: S. cerevisiae RPL39 (P04650; same SF0, PAINT seed, deletion phenotype
PMID:10852723). No review for it exists in genes/yeast/. Module: only modules/dmel_cytoplasmic_ribosome.yaml
(Drosophila RpL39) relates; no fungal ribosome module.