Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Expression cloning of an oxysterol 7alpha-hydroxylase selective for 24-hydroxycholesterol.
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CYP39A1 is a microsomal, liver-expressed cytochrome P450 that is a second oxysterol 7-alpha-hydroxylase (distinct from CYP7B1) with preference for 24-hydroxycholesterol; hepatic expression is sexually dimorphic and not regulated by dietary cholesterol or bile acids.
"CYP39A1 is a microsomal cytochrome P450 enzyme that has preference for 24-hydroxycholesterol and is expressed in the liver"
Genetic, anatomic, and clinical determinants of human serum sterol and vitamin D levels.
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A GWAS plus resequencing/expression/biochemical work at the CYP39A1 locus identified multiple loss-of-function alleles with additive effects on serum levels of 24S-hydroxycholesterol, an established substrate of the encoded enzyme.
"loss-of-function alleles with additive effects on serum levels of the oxysterol, 24S-hydroxycholesterol, a substrate of the encoded enzyme"
Architecture of the human interactome defines protein communities and disease networks.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
CYP39A1 7-hydroxylates 24OH-CHOL
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CYP39A1, located on the ER membrane, 7-alpha-hydroxylates 24-hydroxycholesterol to cholest-5-ene-3beta,7alpha,24-triol; expression is restricted to the liver and it functions in bile acid metabolism.
"24-hydroxycholesterol 7-alpha-hydroxylase (CYP39A1), located on the ER membrane"
Synthesis of bile acids and bile salts via 24-hydroxycholesterol
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Brain-derived 24-hydroxycholesterol is exported to the liver and converted to bile acids/bile salts; this alternative pathway is a minor bile-acid source important for disposing of excess brain cholesterol.
"This pathway is only a minor source of bile acids and bile salts, but appears to be critical for the disposal of excess cholesterol from the brain"
Endogenous sterols
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Several cytochrome P450s participate in cholesterol biosynthesis and elimination and are important for maintaining cholesterol homeostasis.
"A number of CYPs take part in cholesterol biosynthesis and elimination, thus playing an important role in maintaining cholesterol homeostasis"