Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Identification of the sequence encoding N-acetylneuraminate-9-phosphate phosphatase.
-
Identified the gene (HDHD4/NANP) encoding Neu5Ac-9-phosphatase; the recombinant human enzyme is Mg2+-dependent, HAD-family, and >230-fold more efficient on Neu5Ac-9-P than its next-best substrate.
"The recombinant enzyme displayed a"
Design, synthesis, functional and structural characterization of an inhibitor of N-acetylneuraminate-9-phosphate phosphatase: observation of extensive dynamics in an enzyme/inhibitor complex.
-
Structural and functional characterization of HDHD4 (NANP) with a designed phosphonate inhibitor; Km ~47 uM for Neu5Ac-9-P, Mg2+ cofactor, structures with Mg2+ and phosphate (PDB 4KNV/4KNW).
"in the range of the native substrate"
Activity of N-acylneuraminate-9-phosphatase (NANP) is not essential for de novo sialic acid biosynthesis.
-
NANP knockout in human cell lines did not abolish cell-surface sialylation or CMP-sialic acid levels (unlike GNE, NANS, CMAS KO), despite accumulation of the Neu5Ac-9-P substrate, indicating an alternative bypass phosphatase.
"NANP activity is not essential for"
NANP dephosphorylates Neu5Ac-9-P to Neu5Ac