"Both are type II membrane proteins with the C terminus projecting into the lumen of the ER."

"The homology of AADA with hormone-sensitive lipase and the tissue distribution of AADA are consistent with the view that AADA plays a role in promoting the mobilization of lipids from intracellular stores and in the liver for assembling VLDL."

"Previous studies revealed that the luminal orientation of 11beta-HSD1 and 50-kDa esterase/arylacetamide deacetylase (E3) is determined by their highly similar N-terminal transmembrane domains."

"The observation that expression of the human homologue of SAY1 , AADAC, in yeast rescues the sterol acetate accumulation phenotype of say1 Δ-mutant cells indicates that the human aryl acetamide deacetylase acts on cholesterol acetate and thus has overlapping substrate specificity with Say1 in vivo , even though the enzyme has been identified as an N -deacetylase based on its in vitro activity against aryl acetamide ( Probst et al , 1994 )."

"Incubation of HepG2 hepatoma cells with radiolabeled pregnenolone, however, did not reveal any conversion to pregnenolone acetate or export of modified pregnenolone into the culture media, indicating that the substrate specificity of a putative lipid acetylase, if present in mammals, is different from that of yeast cells (data not shown)."

"In the present study, we found that human arylacetamide deacetylase (AADAC) efficiently hydrolyzed flutamide using recombinant AADAC expressed in COS7 cells."

"AADAC is specifically expressed in the endoplasmic reticulum."

"In human liver microsomal samples (n = 50), the flutamide hydrolase activities were significantly correlated with the expression levels of AADAC protein (r = 0.66, p < 0.001)."

"We have established McArdle-RH7777 (rat hepatoma) cell lines stably expressing mouse AADA cDNA and performed metabolic labeling as well as lipid mass analyses."

"Although AADA shares similarity with HSL and like HSL showed hydrolytic activity toward endogenously synthesized DG, we did not observe any in vitro activity of AADA toward membrane-associated TG or CE."

"An acetylaminofluorene deacetylase was purified 90 fold from human liver microsomes by PEG-fractionation, anion exchange and hydrophobic interaction chromatography."

"In conclusion, we found that AADAC is the principal enzyme responsible for the phenacetin hydrolysis, and the difference of hydrolase activity between phenacetin and APAP is largely due to the substrate specificity of AADAC."

"In this study, we found that recombinant human arylacetamide deacetylase (AADAC) could efficiently deacetylate rifamycins, whereas human carboxylesterases, which are enzymes responsible for the hydrolysis of many prodrugs, showed no activity."

"In human, AADAC mRNA was highly expressed in liver and the gastrointestinal tract, followed by bladder."

"High rifampicin hydrolase activity was detected only by recombinant human AADAC and human liver and jejunum microsomes."

"The re-introduction of AADAC to infected cells restored cellular TG lipolysis, indicating a role for HCV-mediated downregulation of AADAC in this process."

"Defective lipolysis of cellular TG stores and VLDL production were also observed in HuH7.5 cells stably expressing a short hairpin RNA targeting AADAC expression, proving AADAC deficiency contributes to these defective pathways."

"This result indicated that AADAC was glycosylated at both N78 and N282."

"Overall, this study found that the translational, but not post-translational, N-glycosylation of AADAC plays a crucial role in regulating AADAC enzyme activity."

"Recombinant AADAC showed a high level of indiplon hydrolase activity, whereas recombinant carboxylesterase 1 (CES1) and 2 (CES2) showed marginal activity."

"Collectively, these results suggest that AADAC prefers compounds with smaller acyl moieties than does CES2."

"Kinetic analysis and inhibition studies using human liver microsomes (HLM) and recombinant enzymes revealed that human arylacetamide deacetylase (AADAC) is responsible for KC hydrolysis to form DAK, and confirmed that FMO3 is the enzyme responsible for DAK N-hydroxylation."

"In conclusion, the present study demonstrated that human AADAC hydrolyzes KC to trigger hepatocellular toxicity."

"Eslicarbazepine acetate was efficiently hydrolyzed by human intestinal and liver microsomes and recombinant human AADAC."

"Recombinant AADAC showed abiraterone acetate hydrolase activity with similar Km value to HIM and HLM."

"Both human and rodent recombinant AADAC proteins can deacetylate NAS in vitro, although the human AADAC shows markedly higher activity compared with rodent enzyme."

"In addition to NAS, recombinant hAADAC can deacetylate melatonin (to form 5-methoxytryptamine) and N-acetyltryptamine (NAT) (to form tryptamine)."

"Here, knockdown of endogenous AADAC in PHH did not alter fatty acid oxidation. However, our negative findings may be due to the modest level of AADAC knockdown and do not preclude a role for this enzyme in metabolism."

"By contrast, AADAC activity did not significantly associate with HOMA-IR nor with DAG species (Figure S4)."

"Overexpression of miR-222-3p resulted in increased lipid accumulation in Huh-1 cells, which was reversed by AADAC overexpression. In contrast, miR-222-3p inhibition decreased lipid accumulation, which was reversed by AADAC knockdown."

"The cDNA was confirmed to be that for DAC in tryptic peptides from the purified human liver protein."

"Highest sequence similarity of DAC was found in a series of prokaryotic esterases encompassing the putative active site."

"The proposed model of the 50-kDa protein predicts one transmembrane segment at the N-terminus, flanked by positively charged residues on the cytosolic surface and negatively charged residues on the lumenal side of the hydrophobic domain, with most of the polypeptide projecting into the lumen of the ER."

"Hydrolases hydrolyse esters, amides, epoxides and glucuronides."

"AADAC hydrolyses PHEN to the p-phenetidine metabolite which is a nephrotoxicant (Watanabe et al. 2010, Fukami & Yokoi 2012)."