Annotation inferences using phylogenetic trees
Characterization of soluble and membrane-bound family 3 lytic transglycosylases from Pseudomonas aeruginosa.
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The lipidated paralog (MltB, PA4444) was truncated before Cys17 to give soluble sMltB.
"Its gene was engineered to produce a truncated form of the enzyme lacking its N-terminal 17 residues which includes Cys17, the putative site of lipidation."
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Purified sMltB and SltB both release anhydromuropeptides from insoluble peptidoglycan.
"Both enzymes produced the same three major soluble products which were identified as anhydromuropeptides based on ESI-MS analysis"
Interaction of penicillin-binding protein 2 with soluble lytic transglycosylase B1 in Pseudomonas aeruginosa.
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MltB is the lipoprotein of the four P. aeruginosa family 3 LTs.
"One, MltB, is a lipoprotein, whereas three other homologues are produced in soluble form"
UniProt entry Q9HVX3 (Q9HVX3_PSEAE)