Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
The RdeA-RegA system, a eukaryotic phospho-relay controlling cAMP breakdown.
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RegA is a two-domain response regulator with an N-terminal receiver domain and a C-terminal cAMP-phosphodiesterase domain.
"The response regulator, RegA, is composed of an N-terminal receiver domain linked to a C-terminal cAMP-phosphodiesterase domain"
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Phosphorylation of the RegA receiver domain activates its phosphodiesterase activity at least 20-fold.
"Phosphorylation of RegA by a heterologous phospho-donor protein activates RegA phosphodiesterase activity at least 20-fold"
The internal phosphodiesterase RegA is essential for the suppression of lateral pseudopods during Dictyostelium chemotaxis.
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RegA is required to suppress lateral pseudopod formation during chemotaxis in increasing temporal cAMP gradients.
"RegA is essential for specifically suppressing lateral pseudopod formation during the response to an increasing temporal gradient of cAMP"
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In regA-null cells myosin II fails to localize normally to the cortex.
"myosin II does not localize in a normal manner to the cortex"
Genetic interactions of the E3 ubiquitin ligase component FbxA with cyclic AMP metabolism and a histidine kinase signaling pathway during Dictyostelium discoideum development.
Adenylyl cyclase G is activated by an intramolecular osmosensor.
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Osmotic up-shift converts DokA to a phosphatase that inactivates RegA, leading to cell cortex fortification.
"This results in inactivation of the cAMP phosphodiesterase RegA and ultimately in fortification of the cell cortex"
cAMP controls cytosolic Ca2+ levels in Dictyostelium discoideum.
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The regA-null mutant lacks the main cAMP phosphodiesterase and shows altered cAMP metabolism.
"a mutant that is devoid of the main cAMP-phosphodiesterase (PDE) RegA and displays an altered cAMP metabolism"
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Loss of RegA is associated with augmented release of Ca2+ from internal stores.
"an augmented release of Ca2+ from stores in the mutant"
Seven Dictyostelium discoideum phosphodiesterases degrade three pools of cAMP and cGMP.
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RegA (DdPDE2) is a cAMP-specific PDE localized in the cytosol that degrades intracellular cAMP and is regulated by histidine kinase/phosphorelay.
"DdPDE2 (RegA) encodes a cAMP-specific PDE localized in the cytosol of the cell where it degrades intracellular cAMP"
High-throughput analysis of spatio-temporal dynamics in Dictyostelium.
Loss of cAMP-specific phosphodiesterase rescues spore development in G protein mutant in dictyostelium.
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Loss of RegA accelerates spore development, consistent with elevated cAMP/PKA driving precocious differentiation.
"Loss of RegA also results in accelerated spore development"
Response of Dictyostelium discoideum to UV-C and involvement of poly (ADP-ribose) polymerase.
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regA was among developmentally regulated genes monitored by RT-PCR after UV-C exposure.
"regA, ctnA, ctnB, gp24, hspD and dsn were analysed using semiquantitative RT-PCR"
Differentiation-inducing factor 2 modulates chemotaxis via the histidine kinase DhkC-dependent pathway in Dictyostelium discoideum.
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DIF-2 enhances chemotaxis in shallow cAMP gradients via RegA within the DhkC-RdeA-RegA two-component system.
"DIF-2 enhances chemotaxis under the same conditions via a phosphodiesterase, response regulator A (RegA)"
Mitogen-activated protein kinase regulation of the phosphodiesterase RegA in early Dictyostelium development.
A cAMP-phosphodiesterase controls PKA-dependent differentiation.
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RegA phosphodiesterase is stimulated by binding to the PKA regulatory subunit (PKA-R).
"stimulated by binding to the regulatory subunit of cAMP-dependent protein kinase, PKA-R"
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Inhibition of RegA increases PKA activity at a terminal differentiation checkpoint.
"inhibition of the phosphodiesterase results in an increase in the activity of PKA, which acts at a check point for terminal differentiation"
An intersection of the cAMP/PKA and two-component signal transduction systems in Dictyostelium.
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RegA has a cAMP phosphodiesterase domain (Km ~5 uM) and a response-regulator domain; phosphotransfer depends on Asp212.
"One domain is a cAMP phosphodiesterase (Km approximately 5 microM); the other is homologous to response regulators"
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Phosphotransfer to RegA is dependent on Asp212, the predicted phosphoacceptor.
"with transfer dependent on Asp212, the predicted phosphoacceptor"
Identification and characterization of two unusual cGMP-stimulated phoshodiesterases in dictyostelium.
Development of Phosphodiesterase-Protein-Kinase Complexes as Novel Targets for Discovery of Inhibitors with Enhanced Specificity.
OpenScientist focused regA GO:0047555 hypothesis report
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Report recommendation was critically incorporated; the disputed molecular/process claim remains UNDECIDED because the report does not resolve the decisive primary evidence.
"no primary paper reports cGMP as a substrate"