FLVCR1 source and context guide

All 68 original QuickGO contexts map one-to-one to 68 genuine seed rows. The review preserves both natural products, original source objects/order and three explicitly restored singleton extensions. Counts: 17 ACCEPT, 23 KEEP_AS_NON_CORE, 10 MODIFY, 13 UNDECIDED, 3 TOO_GENERAL, 2 MARK_AS_OVER_ANNOTATED; two cores, 21 references, zero NEW/quotes.

Original context mapping

Stored row (0-based)	Raw index	Context ID	Term	Evidence	Reference	Assigned by	Partners	Original extensions	Decision
0	3	UniProtKB:Q9Y5Y0!477464500	GO:0005515	IPI	PMID:20610401	UniProt	UniProtKB:P02790	null	KEEP_AS_NON_CORE
1	4	UniProtKB:Q9Y5Y0!477464501	GO:0005515	IPI	PMID:36115835	IntAct	UniProtKB:A4D2P6	null	UNDECIDED
2	5	UniProtKB:Q9Y5Y0!477464502	GO:0005515	IPI	PMID:36115835	IntAct	UniProtKB:O75970	null	UNDECIDED
3	6	UniProtKB:Q9Y5Y0!477464503	GO:0005515	IPI	PMID:36115835	IntAct	UniProtKB:P78352	null	MODIFY
4	7	UniProtKB:Q9Y5Y0!477464504	GO:0005515	IPI	PMID:36115835	IntAct	UniProtKB:Q02410	null	UNDECIDED
5	8	UniProtKB:Q9Y5Y0!477464505	GO:0005515	IPI	PMID:36115835	IntAct	UniProtKB:Q07157	null	UNDECIDED
6	9	UniProtKB:Q9Y5Y0!477464506	GO:0005515	IPI	PMID:36115835	IntAct	UniProtKB:Q14005	null	UNDECIDED
7	10	UniProtKB:Q9Y5Y0!477464507	GO:0005515	IPI	PMID:36115835	IntAct	UniProtKB:Q14160	null	MODIFY
8	11	UniProtKB:Q9Y5Y0!477464508	GO:0005515	IPI	PMID:36115835	IntAct	UniProtKB:Q15700	null	UNDECIDED
9	12	UniProtKB:Q9Y5Y0!477464509	GO:0005515	IPI	PMID:36115835	IntAct	UniProtKB:Q5T2W1	null	MODIFY
10	13	UniProtKB:Q9Y5Y0!477464510	GO:0005515	IPI	PMID:36115835	IntAct	UniProtKB:Q68DX3	null	UNDECIDED
11	14	UniProtKB:Q9Y5Y0!477464511	GO:0005515	IPI	PMID:36115835	IntAct	UniProtKB:Q86UL8	null	MODIFY
12	15	UniProtKB:Q9Y5Y0!477464512	GO:0005515	IPI	PMID:36115835	IntAct	UniProtKB:Q86UT5	null	MODIFY
13	16	UniProtKB:Q9Y5Y0!477464513	GO:0005515	IPI	PMID:36115835	IntAct	UniProtKB:Q8N448	null	UNDECIDED
14	17	UniProtKB:Q9Y5Y0!477464514	GO:0005515	IPI	PMID:36115835	IntAct	UniProtKB:Q8NI35	null	UNDECIDED
15	18	UniProtKB:Q9Y5Y0!477464515	GO:0005515	IPI	PMID:36115835	IntAct	UniProtKB:Q8TBB1	null	MODIFY
16	19	UniProtKB:Q9Y5Y0!477464516	GO:0005515	IPI	PMID:36115835	IntAct	UniProtKB:Q92796	null	MODIFY
17	20	UniProtKB:Q9Y5Y0!477464517	GO:0005515	IPI	PMID:36115835	IntAct	UniProtKB:Q96QZ7	null	UNDECIDED
18	21	UniProtKB:Q9Y5Y0!477464518	GO:0005515	IPI	PMID:36115835	IntAct	UniProtKB:Q99767	null	UNDECIDED
19	22	UniProtKB:Q9Y5Y0!477464519	GO:0005515	IPI	PMID:36115835	IntAct	UniProtKB:Q9C0E4	null	UNDECIDED
20	23	UniProtKB:Q9Y5Y0!477464520	GO:0005515	IPI	PMID:36115835	IntAct	UniProtKB:Q9P202	null	MODIFY
21	24	UniProtKB:Q9Y5Y0!477464521	GO:0005515	IPI	PMID:36115835	IntAct	UniProtKB:Q9Y3R0	null	UNDECIDED
22	55	UniProtKB:Q9Y5Y0!477464552	GO:0005739	IBA	GO_REF:0000033	GO_Central	MGI:MGI:2444881; PANTHER:PTN002589204; UniProtKB:Q9Y5Y0	null	KEEP_AS_NON_CORE
23	60	UniProtKB:Q9Y5Y0!477464557	GO:0005739	IDA	PMID:23187127	MGI		null	KEEP_AS_NON_CORE
24	61	UniProtKB:Q9Y5Y0!477464558	GO:0005743	IEA	GO_REF:0000117	UniProt	ARBA:ARBA00028608	null	MODIFY
25	62	UniProtKB:Q9Y5Y0!477464559	GO:0005743	TAS	Reactome:R-HSA-9661408	Reactome		null	MODIFY
26	64	UniProtKB:Q9Y5Y0!477464561	GO:0005886	EXP	PMID:15369674	UniProt		null	ACCEPT
27	56	UniProtKB:Q9Y5Y0!477464553	GO:0005886	IDA	PMID:22483575	UniProt		null	ACCEPT
28	57	UniProtKB:Q9Y5Y0!477464554	GO:0005886	IDA	PMID:37100056	UniProt		null	ACCEPT
29	58	UniProtKB:Q9Y5Y0!477464555	GO:0005886	IDA	PMID:38778100	UniProt		null	ACCEPT
30	63	UniProtKB:Q9Y5Y0!477464560	GO:0005886	IEA	GO_REF:0000120	UniProt	ARBA:ARBA00027801; UniProtKB-SubCell:SL-0039	null	ACCEPT
31	65	UniProtKB:Q9Y5Y0!477464562	GO:0005886	TAS	Reactome:R-HSA-917892	Reactome		null	ACCEPT
32	42	UniProtKB:Q9Y5Y0!477464539	GO:0006783	IEA	GO_REF:0000117	UniProt	ARBA:ARBA00027909	null	MARK_AS_OVER_ANNOTATED
33	43	UniProtKB:Q9Y5Y0!477464540	GO:0006783	TAS	Reactome:R-HSA-189451	Reactome		null	MARK_AS_OVER_ANNOTATED
34	44	UniProtKB:Q9Y5Y0!477464541	GO:0006839	IBA	GO_REF:0000033	GO_Central	PANTHER:PTN002589204; UniProtKB:Q9Y5Y0	null	KEEP_AS_NON_CORE
35	0	UniProtKB:Q9Y5Y0!477464497	GO:0006839	IDA	PMID:23187127	MGI		null	KEEP_AS_NON_CORE
36	45	UniProtKB:Q9Y5Y0!477464542	GO:0006879	TAS	Reactome:R-HSA-917937	Reactome		null	KEEP_AS_NON_CORE
37	46	UniProtKB:Q9Y5Y0!477464543	GO:0008654	IDA	PMID:38693265	UniProt		null	KEEP_AS_NON_CORE
38	25	UniProtKB:Q9Y5Y0!477464522	GO:0015220	IDA	PMID:37100056	UniProt		null	ACCEPT
39	26	UniProtKB:Q9Y5Y0!477464523	GO:0015220	IDA	PMID:38693265	UniProt		[{"connectedXrefs":[{"db":"GO","id":"0008654","relation":"part_of"}]}]	ACCEPT
40	27	UniProtKB:Q9Y5Y0!477464524	GO:0015220	IDA	PMID:38778100	UniProt		null	ACCEPT
41	29	UniProtKB:Q9Y5Y0!477464526	GO:0015220	IDA	PMID:40972734	UniProt		null	ACCEPT
42	28	UniProtKB:Q9Y5Y0!477464525	GO:0015220	IMP	PMID:39306721	UniProt		null	ACCEPT
43	30	UniProtKB:Q9Y5Y0!477464527	GO:0015232	IBA	GO_REF:0000033	GO_Central	MGI:MGI:2444881; PANTHER:PTN000858905; UniProtKB:Q9UPI3; UniProtKB:Q9Y5Y0	null	KEEP_AS_NON_CORE
44	33	UniProtKB:Q9Y5Y0!477464530	GO:0015232	IDA	PMID:20610401	UniProt		null	KEEP_AS_NON_CORE
45	34	UniProtKB:Q9Y5Y0!477464531	GO:0015232	IDA	PMID:22483575	UniProt		[{"connectedXrefs":[{"db":"GO","id":"0097037","relation":"part_of"}]}]	KEEP_AS_NON_CORE
46	31	UniProtKB:Q9Y5Y0!477464528	GO:0015232	IEA	GO_REF:0000117	UniProt	ARBA:ARBA00027969	null	KEEP_AS_NON_CORE
47	32	UniProtKB:Q9Y5Y0!477464529	GO:0015232	IMP	PMID:15369674	UniProt		null	KEEP_AS_NON_CORE
48	35	UniProtKB:Q9Y5Y0!477464532	GO:0015232	TAS	Reactome:R-HSA-917892	Reactome		null	KEEP_AS_NON_CORE
49	36	UniProtKB:Q9Y5Y0!477464533	GO:0015232	TAS	Reactome:R-HSA-9661408	Reactome		null	KEEP_AS_NON_CORE
50	47	UniProtKB:Q9Y5Y0!477464544	GO:0015871	IDA	PMID:38778100	UniProt		null	ACCEPT
51	48	UniProtKB:Q9Y5Y0!477464545	GO:0015871	IDA	PMID:40972734	UniProt		null	ACCEPT
52	1	UniProtKB:Q9Y5Y0!477464498	GO:0015886	IMP	PMID:23187127	MGI		null	KEEP_AS_NON_CORE
53	59	UniProtKB:Q9Y5Y0!477464556	GO:0016020	IBA	GO_REF:0000033	GO_Central	FB:FBgn0033196; MGI:MGI:2384974; MGI:MGI:2444881; PANTHER:PTN000858822; UniProtKB:Q6GNV7; UniProtKB:Q96SL1; UniProtKB:Q9UPI3; UniProtKB:Q9Y5Y0	null	TOO_GENERAL
54	37	UniProtKB:Q9Y5Y0!477464534	GO:0020037	IBA	GO_REF:0000033	GO_Central	PANTHER:PTN000858905; UniProtKB:Q9UPI3	null	KEEP_AS_NON_CORE
55	38	UniProtKB:Q9Y5Y0!477464535	GO:0022857	IEA	GO_REF:0000002	InterPro	InterPro:IPR011701; InterPro:IPR020846	null	TOO_GENERAL
56	49	UniProtKB:Q9Y5Y0!477464546	GO:0030218	IBA	GO_REF:0000033	GO_Central	MGI:MGI:2444881; PANTHER:PTN002589204; UniProtKB:Q9Y5Y0; ZFIN:ZDB-GENE-041014-359	null	KEEP_AS_NON_CORE
57	2	UniProtKB:Q9Y5Y0!477464499	GO:0030218	IDA	PMID:23187127	MGI		null	KEEP_AS_NON_CORE
58	50	UniProtKB:Q9Y5Y0!477464547	GO:0030218	IEA	GO_REF:0000117	UniProt	ARBA:ARBA00085625	null	KEEP_AS_NON_CORE
59	67	UniProtKB:Q9Y5Y0!477464564	GO:0031966	EXP	PMID:23187127	UniProt		null	KEEP_AS_NON_CORE
60	66	UniProtKB:Q9Y5Y0!477464563	GO:0031966	IEA	GO_REF:0000044	UniProt	UniProtKB-SubCell:SL-0171	null	KEEP_AS_NON_CORE
61	39	UniProtKB:Q9Y5Y0!477464536	GO:0034228	IDA	PMID:38693265	UniProt		[{"connectedXrefs":[{"db":"GO","id":"0008654","relation":"part_of"}]}]	ACCEPT
62	40	UniProtKB:Q9Y5Y0!477464537	GO:0034228	IDA	PMID:38778100	UniProt		null	ACCEPT
63	41	UniProtKB:Q9Y5Y0!477464538	GO:0034228	IMP	PMID:39306721	UniProt		null	ACCEPT
64	51	UniProtKB:Q9Y5Y0!477464548	GO:0034229	IEA	GO_REF:0000108	GOC	GO:0034228	null	ACCEPT
65	52	UniProtKB:Q9Y5Y0!477464549	GO:0055085	IEA	GO_REF:0000002	InterPro	InterPro:IPR011701	null	TOO_GENERAL
66	53	UniProtKB:Q9Y5Y0!477464550	GO:0097037	IBA	GO_REF:0000033	GO_Central	PANTHER:PTN000858905; UniProtKB:Q9Y5Y0	null	KEEP_AS_NON_CORE
67	54	UniProtKB:Q9Y5Y0!477464551	GO:0097037	IMP	PMID:15369674	UniProt		null	KEEP_AS_NON_CORE

The three nonempty extensions each contain one disjunct with one conjunct. YAML extensions use predicate BFO:0000050 (part of) and the exact original term. No OR/conjunction semantics are lost; no inference is taken from the TSV annotation_extension column. The source JSON remains unchanged.

Normal source closure

Nine exact-main sources were hydrated before genuine acquisition of eight absent sources. All 17 normal files are immutable outputs, with no provider-text fabrication.

Source path	Bytes	SHA256
interpro/panther/PTHR10924/PTHR10924-entries.csv	3489	8b94e935c03f439529c18ca060799a4bdc0eb489f8c80424cf04fdc12d0fe342
interpro/panther/PTHR10924/PTHR10924-metadata.yaml	954	6dd9f6ec7363c0ab878aa4050b9183f0d977bb85651701653053701d36a6130c
interpro/panther/PTHR10924/PTHR10924-paint.tsv	934	16d069553243a627ce7e4a1522ee3e7a90afe20da6bf12da30f9ccb222b92f78
publications/PMID_15369674.md	2139	f6c97b07acd5fb97c9ed1c2b8a93943fbef585c687e4100273bc2f3761fbe165
publications/PMID_20610401.md	18070	e4b100c4d7a7993287e7e576a373be874d1c7dfec1ba60602b00ebfb990acdf2
publications/PMID_22483575.md	2668	0ea4b3c39536d799867c418281147e6bd3b201088d7a6bff28d60a0edf5fe2b1
publications/PMID_23187127.md	40816	e7db459807e9be2ccf2d883e8c0bc1e5662c48aae38bedd244e5139a82591e5c
publications/PMID_36115835.md	302401	a8561380015e9e8bbc463d3e9efd1eff0e88d345d9bfea98e47b2bb94e6f698c
publications/PMID_37100056.md	228109	d83ba63f75c1b182b67bd62ca42f6fbee533eb32a174c90a036ac78f5d3f5c0e
publications/PMID_38693265.md	185330	b5f97d969d2e780daa21548650e60c3a9ee5056a5ae5eba2c5e91c64391d44ce
publications/PMID_38778100.md	145945	99b5a14b48d2e4226d214b2f8de5ff01b0250ee4d918b67b170aa299a70f1e30
publications/PMID_39306721.md	12714	9b20f13793fff33e7f87b31dc22ffa055667a3bb8817a089990e9dd613a73983
publications/PMID_40972734.md	3454	aed5099079cd893498447e206987f18c3842e357acb4a330d5c38ce982643238
reactome/R-HSA-189451.md	3799	c2f73bcaada2b5d149ba045f7ea206b71a4e9d20606d49782d245ac62ba4fcdc
reactome/R-HSA-917892.md	1959	ed6e0a93f39d25f3c044ad0d568fc97bcaf5bc5eeb654a4cf596dc0c0e301e55
reactome/R-HSA-917937.md	1227	3c473a8c97f41a1c98890322952ff6c0f6022096e1861cac2ee27655b4e64d5d
reactome/R-HSA-9661408.md	1074	b18be081998d9b00383accee9d2e0d0db8ec0fdb4378d937c11af1194593e156

Actual source access

Cache availability and what was actually read are distinct. No figure-pixel or uninspected supplement claim is made.

PMID:15369674(https://pubmed.ncbi.nlm.nih.gov/15369674/) — Authentic normal abstract and selected original publisher-PDF Results/Methods read. Human AF118637 FLVCR cDNA in rat NRK cells supports ZnMP and radiolabeled-hemin washout and extracellular HPLC-detected heme, with separate human K562 interference experiments. This is cellular export, not purified-transporter reconstitution or a short-product assay. No figure-pixel audit. Normal full-text flag: false.
PMID:20610401(https://pubmed.ncbi.nlm.nih.gov/20610401/) — Selected original full-text Methods/Results and Fig7 caption read. Human FLVCR expression in rat cells and acceptor-dependent cellular export are distinct from binding of a GST-tagged FLVCR fragment spanning residues 132–201 to purified rabbit apohemopexin. The original Methods and Results both specify residues 132–201, whereas the Discussion gives 132–301; the normal cache retains that conflicting Discussion range. The review follows the Methods/Results description while recording this internal discrepancy; the construct sequence has not been independently resolved. The fragment insert species is not separately specified in the inspected Methods. No full-length human-human affinity or complete histidine mechanism is established. Normal full-text flag: true.
PMID:22483575(https://pubmed.ncbi.nlm.nih.gov/22483575/) — Authentic abstract verified; full original Methods remain unavailable. It reports variant loss of fluorescent-heme-analog export, trafficking defects and reduced stability. Original host species and individual assay details are not invented; independent human-protein studies support retained function and location. Normal full-text flag: false.
PMID:23187127(https://pubmed.ncbi.nlm.nih.gov/23187127/) — Selected original full-text Results/Methods read. Endogenous human mitochondrial-fraction immunoblot and human transcript depletion are distinct from tagged murine Flvcr1a/b constructs in human HeLa/K562 cells. Compartmental heme-content and differentiation endpoints support contextual roles, not purified short-product translocation or inner-versus-outer membrane resolution. No figure-pixel or separate supplement audit. Normal full-text flag: true.
PMID:36115835(https://pubmed.ncbi.nlm.nih.gov/36115835/) — Authentic original Methods/Results, exact IntAct pairs and original SupplementaryDataset1 measurements were read and joined. Eight source pairs have above-threshold domain-peptide measurements; thirteen remain unresolved because every tested partner domain is below the authors detection threshold. Citation identity and measurements are verified, but those thirteen positive GOA assertions are not independently verified. No false nonbinding conclusion, invented affinity, full-length complex claim or FLVCR1-specific FP replication. Normal full-text flag: true.
PMID:37100056(https://pubmed.ncbi.nlm.nih.gov/37100056/) — Selected original full-text Results/Methods read. Human-cell loss and rescue, radiolabeled choline uptake and unchanged measured choline-kinase activity distinguish transport from downstream phosphorylation. Full-length product rescue and paired tagged localization Methods are read; supplementary image pixels were not independently audited. Mouse phenotypes remain separate. Normal full-text flag: true.
PMID:38693265(https://pubmed.ncbi.nlm.nih.gov/38693265/) — Final original Nature article is now genuinely available in the normal full-text cache. Selected Results, protein-expression, proteoliposome-reconstitution/uptake and cellular-assay Methods read. Purified human choline translocation is distinct from cellular ethanolamine uptake, selective mutagenesis and isotope tracing into phosphoethanolamine. Unresolved atomic-model termini are not natural-product deletions. No figure-pixel or separate supplement audit. Normal full-text flag: true.
PMID:38778100(https://pubmed.ncbi.nlm.nih.gov/38778100/) — Selected original full-text Results/Methods read. Human full-length FLVCR1a uptake, membrane imaging and substrate-bound structures support the two activities. CHKA/ETNK1 enhance intracellular trapping; cellular accumulation is not purified turnover. FLVCR1 ethanolamine washout is distinct from FLVCR2 choline washout. Transport is facilitative rather than an ATP pump or obligatory one-way importer. Normal full-text flag: true.
PMID:39306721(https://pubmed.ncbi.nlm.nih.gov/39306721/) — Authentic normal abstract and previously recovered final publisher selected Methods/Results read in the independent consultation. Human FLVCR1/variant assays in HEK293 cells use CHKA or ETNK1 and kinase-only controls. Coupled uptake/trapping endpoints corroborate transport; no purified-variant kinetics, universal disease-severity inference or earlier-preprint substitution. Normal full-text flag: false.
PMID:40972734(https://pubmed.ncbi.nlm.nih.gov/40972734/) — Authentic identifier, title and abstract verified. The exact FLVCR1 comparison/assay remains inaccessible; the abstract does not enumerate all sixteen targets. Existing choline function/location is supported independently, but other SLC kinetics or inhibitor data are not assigned to FLVCR1 and this source-specific claim remains unverified. Normal full-text flag: false.

IBA origins and product identity

The complete 359-node family tree and 359-record alignment identify human Q9Y5Y0 at PTN002475065 with the 555-residue sequence. All eight normal PAINT rows match the original IBD GAF. The seven incoming origins below are positive and on the actual target path; the remaining family assertion is not manufactured as a NEW target annotation.

Raw index	Term	Origin	Taxonomic range	On target path
30	GO:0015232	PTN000858905	Eumetazoa	true
37	GO:0020037	PTN000858905	Eumetazoa	true
44	GO:0006839	PTN002589204	Euteleostomi	true
49	GO:0030218	PTN002589204	Euteleostomi	true
53	GO:0097037	PTN000858905	Eumetazoa	true
55	GO:0005739	PTN002589204	Euteleostomi	true
59	GO:0016020	PTN000858822	Eukaryota	true

PANTHER family, original IBD GAF, official UniProt. Official FASTAs reconstruct Q9Y5Y0-1 (555 aa) and Q9Y5Y0-2 (279 aa), with the latter lacking residues 1–276. Product sequence identity does not transfer localization, transport direction or substrate repertoire.

The choline and ethanolamine activities are distinct and have part_of relations to their respective transport processes. Their ancestry covers generic transmembrane transport; plasma membrane entails membrane. PDZ domain binding is supported by the actual domain-peptide measurements. Mitochondrial membrane preserves supported localization while removing untested inner-bilayer specificity.

Review input provenance

Private original-data and consultation records are retained locally. These hashes identify the exact accepted inputs without requiring public access to a local absolute path.

FLVCR1-root-complete-matrix-20261028-v1/decision-matrix-v2.json: 179e99c275e67b9b1e816e82769fc0730b70d81553ff414d7b28d43dd94fb055
FLVCR1-root-complete-matrix-20261028-v1/source-scope-and-core-proposal-v2.json: c0a988b5eb0d837e598098c5bac562ab1c301c14012c769e275e452a17ff482d
FLVCR1-root-complete-matrix-20261028-v1/root-specificity-acceptance-v2.json: dbb55748d8066b5a4ed658325fb4ab9f1e1e9189892afc72495d9e3dd46367a6
FLVCR-extensions-plan-tracker642-20261028-v1/serialization-plan.json: 00c38e1aff9fc9ae2b39f78c30b4d577b700707006793db41926e356bdb9ff62
FLVCR-extensions-plan-tracker642-20261028-v1/root-plan-acceptance.json: 90892e242ef48b02a2a8f96640394aa1da22c615a26c4ac6bcb567c4e5e1cae9
FLVCR1-core-scope-peer-tracker642-20261028-v1/peer-review.json: bba3b382215f68fb839b4eeefc0e34204a719cc2d44f5a038382fb15ffeba213
FLVCR1-intake-root-20261028-v1/phylogeny-proof.json: 688361ce85cb7825507088ee58eae499030d1e27078bacafe3e7969227cd3d76
FLVCR1-heme-fhod3-20261028-v1/HANDOFF-v2.json: 73f53990132f58f6a9c8d87fe5fb213a1ed53790a209ba3600c2fb0159b0521c
FLVCR1-PDZ-specificity-peer-tracker642-20261028-v1/peer-review.json: b87226fa46997d278a759eaa3ef9a4137ee4f6f91c2eae243ff40e235a6f5fd1
FLVCR1-review-20261028-v1/FLVCR1-ai-review.yaml.seed: 6197210b6f2f3140c66444efd3b52a45a3999ba9e9356e4412973ccaf83c58d6
FLVCR1-review-20261028-v1/context-projection-proof.json: e36868e2a8267eeb70c090b58a614c0e9fe82332fd6db5c93886bdb1467570a7

PR5716 review clarification

Thirteen existing source-pair uncertainties are now recorded as OTHER / INTACT / SUSPECTED / CHECK, with no proposed correction or deletion and no upstream-report claim. Their original UNDECIDED actions and source contexts remain intact.

The phospholipid-pathway rationale distinguishes FLVCR1-mediated precursor entry, experimentally coupled to downstream precursor labeling and represented by original part_of extensions, from export or redistribution of already completed heme. It does not claim phospholipid catalysis, mature-lipid tracing, a universally committed transport step or a unique precursor route. Both mitochondrial-membrane replacements explicitly acknowledge the two pre-existing GO:0031966 assertions.

PMID:37100056 found no heme/iron effect on choline uptake or incorporation and no heme-accumulation transcriptional/genetic signature in its tested system; its authors explicitly leave other physiological substrates and erythroid-specific contexts open. In PMID:38693265, the size of an observed co-purified endogenous ligand density excludes heme as that density's identity, rather than excluding every possible heme substrate or mechanism. These observations bound the interpretation of the older cellular heme results; they do not establish that those context-specific measurements are false or require blanket DISPUTED reference states.

Auditable PDZ measurement split

This table is a derived extract of PMID:36115835 Supplementary Dataset1, original workbook SHA256 ada912921164f0a79630c6a714b9ff264a7217ad3e646153ef498eb456206795. It includes every tested domain row for the 21 original GOA partner accessions. The retained complete FLVCR1 export contains 133 rows; this partner-specific selection contains 68 rows. Excel row locators are one-based and include the workbook header rows. All Excel row locators below refer to the workbook sheet PDZ-PBM interactome. Partner aliases below are the names in the original dataset, joined to the immutable GOA accessions.

All selected measurements use the FLVCR1 C-terminal peptide MLSKQSESAI, DAPF singlicates (n=1), and an estimated peptide concentration of 18 µM for affinity conversion; no pair-specific fluorescence-polarization measurement is present. The original DAPF Methods define a BI threshold of 0.05 (2σ, σ=0.025). The split uses normalized DAPF BI ≥0.05 in at least one tested domain. Composite pKd=0 is the dataset code on the below-threshold rows, not a measured zero affinity. LNX1_1 is only slightly above threshold; the table does not turn this into robust or high-affinity binding.

Raw / stored	Partner / dataset alias	Tested / above threshold	Domain: normalized BI; composite pKd; Excel row	Action
4 / 1	A4D2P6 / GRID2IP	2 / 0	GRID2IP_2: -0.00543856; 0.00000000; row 48877; GRID2IP_1: 0.00540490; 0.00000000; row 53187	UNDECIDED
5 / 2	O75970 / MPDZ	3 / 0	MPDZ_10: -0.00568999; 0.00000000; row 48767; MPDZ_5: 0.01819104; 0.00000000; row 57809; MPDZ_12: 0.04130598; 0.00000000; row 63725	UNDECIDED
6 / 3	P78352 / DLG4	3 / 1	DLG4_2: 0.10857815; 3.84099400; row 11634; DLG4_1: -0.06998567; 0.00000000; row 36230; DLG4_3: 0.03646020; 0.00000000; row 62812	MODIFY
7 / 4	Q02410 / APBA1	2 / 0	APBA1_1: 0.00227827; 0.00000000; row 51952; APBA1_2: 0.00471480; 0.00000000; row 52911	UNDECIDED
8 / 5	Q07157 / TJP1	2 / 0	TJP1_3: -0.00798629; 0.00000000; row 47850; TJP1_2: 0.03632779; 0.00000000; row 62787	UNDECIDED
9 / 6	Q14005 / IL16	3 / 0	IL16_4: 0.01435211; 0.00000000; row 56502; IL16_3: 0.02697970; 0.00000000; row 60410; IL16_1: 0.04247018; 0.00000000; row 63920	UNDECIDED
10 / 7	Q14160 / SCRIB	3 / 3	SCRIB_3: 0.40150376; 4.61194366; row 3713; SCRIB_1: 0.12521623; 3.91274333; row 10596; SCRIB_2: 0.05921213; 3.54939895; row 16633	MODIFY
11 / 8	Q15700 / DLG2	2 / 0	DLG2_3: 0.02816102; 0.00000000; row 60754; DLG2_2: 0.04432872; 0.00000000; row 64215	UNDECIDED
12 / 9	Q5T2W1 / NHERF3	3 / 1	NHERF3_1: 0.10244406; 3.81215387; row 12070; NHERF3_3: 0.00998088; 0.00000000; row 54942; NHERF3_4: 0.03430271; 0.00000000; row 62330	MODIFY
13 / 10	Q68DX3 / FRMPD2	3 / 0	FRMPD2_1: -0.01184627; 0.00000000; row 46400; FRMPD2_2: 0.00662011; 0.00000000; row 53640; FRMPD2_3: 0.03235218; 0.00000000; row 61863	UNDECIDED
14 / 11	Q86UL8 / MAGI2	5 / 2	MAGI2_2: 0.09935395; 3.79705456; row 12291; MAGI2_4: 0.05750038; 3.53570213; row 16902; MAGI2_5: -0.00492078; 0.00000000; row 49095; MAGI2_6: -0.00473185; 0.00000000; row 49171; MAGI2_3: 0.04034137; 0.00000000; row 63563	MODIFY
15 / 12	Q86UT5 / NHERF4	3 / 2	NHERF4_4: 0.12730031; 3.92115500; row 10482; NHERF4_1: 0.06574312; 3.59850318; row 15653; NHERF4_2: -0.02680831; 0.00000000; row 41546	MODIFY
16 / 13	Q8N448 / LNX2	2 / 0	LNX2_3: -0.00746177; 0.00000000; row 48061; LNX2_2: 0.00560608; 0.00000000; row 53265	UNDECIDED
17 / 14	Q8NI35 / INADL	9 / 0	Inadl_2: -0.01960264; 0.00000000; row 43684; Inadl_1: -0.01170000; 0.00000000; row 46468; Inadl_6: -0.00762139; 0.00000000; row 47985; Inadl_3: -0.00561491; 0.00000000; row 48795; Inadl_4: -0.00469593; 0.00000000; row 49190; Inadl_9: 0.00131268; 0.00000000; row 51568; Inadl_10: 0.01333431; 0.00000000; row 56134; Inadl_5: 0.02112839; 0.00000000; row 58776; Inadl_8: 0.02807916; 0.00000000; row 60723	UNDECIDED
18 / 15	Q8TBB1 / LNX1	3 / 1	LNX1_1: 0.05565566; 3.52051124; row 17226; LNX1_2: -0.01089871; 0.00000000; row 46750; LNX1_3: -0.01001789; 0.00000000; row 47097	MODIFY
19 / 16	Q92796 / DLG3	3 / 1	DLG3_2: 0.07809329; 3.68025717; row 14181; DLG3_3: -0.00508573; 0.00000000; row 49027; DLG3_1: 0.01742691; 0.00000000; row 57532	MODIFY
20 / 17	Q96QZ7 / MAGI1	2 / 0	MAGI1_2: 0.02893391; 0.00000000; row 60970; MAGI1_1: 0.04514768; 0.00000000; row 64355	UNDECIDED
21 / 18	Q99767 / APBA2	2 / 0	APBA2_2: -0.01387088; 0.00000000; row 45615; APBA2_1: 0.02733613; 0.00000000; row 60519	UNDECIDED
22 / 19	Q9C0E4 / GRIP2	5 / 0	GRIP2_6: -0.00570373; 0.00000000; row 48753; GRIP2_3: -0.00044687; 0.00000000; row 50850; GRIP2_4: 0.01022129; 0.00000000; row 55037; GRIP2_5: 0.01054103; 0.00000000; row 55163; GRIP2_7: 0.02200554; 0.00000000; row 59022	UNDECIDED
23 / 20	Q9P202 / DFNB31	3 / 1	DFNB31_1: 0.53587468; 4.86222061; row 2360; DFNB31_2: -0.00552728; 0.00000000; row 48839; DFNB31_3: 0.01608469; 0.00000000; row 57094	MODIFY
24 / 21	Q9Y3R0 / GRIP1	5 / 0	GRIP1_2: -0.11892624; 0.00000000; row 34903; GRIP1_4: -0.00691835; 0.00000000; row 48277; GRIP1_3: -0.00346795; 0.00000000; row 49669; GRIP1_7: 0.00284319; 0.00000000; row 52186; GRIP1_5: 0.01230053; 0.00000000; row 55758	UNDECIDED

The eight above-threshold source pairs retain their PDZ-domain-binding refinements. The thirteen remaining assertions stay UNDECIDED with suspected source-record checks for IntAct; these flags do not establish nonbinding, an incorrect IPI code or a missing NOT qualifier. No upstream report has been filed. The table reproduces the original measurement split without transferring isolated peptide/domain behavior to full-length cellular complexes.