Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Sensitization of Dictyostelium chemotaxis by phosphoinositide-3-kinase-mediated self-organizing signalling patches.
Chemotaxis: signalling the way forward.
A cell number counting factor regulates Akt/protein kinase B to regulate Dictyostelium discoideum group size.
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CRAC translocation between cytosol and membranes was directly assayed by fractionation and GFP-CRAC imaging.
"there is a quantitative change in the translocation of CRAC in the presence of anti-countin antibodies or recombinant countin"
Chemoattractant signaling in dictyostelium discoideum.
Quantitative imaging of single live cells reveals spatiotemporal dynamics of multistep signaling events of chemoattractant gradient sensing in Dictyostelium.
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PH(Crac)-GFP translocation from cytosol to the leading-front plasma membrane reports PIP3 dynamics during gradient sensing.
"PH domain-containing proteins translocate from the cytosol to the plasma membrane at the leading front"
The PI3K-mediated activation of CRAC independently regulates adenylyl cyclase activation and chemotaxis.
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CRAC is essential for chemoattractant-mediated activation of adenylyl cyclase ACA and is separately required for chemotaxis.
"in addition to its essential role in the activation of ACA, CRAC is involved in regulating chemotaxis"
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A CRAC mutant unable to bind PI3K products loses chemotaxis and ACA activation, showing PIP3 binding is required for CRAC function.
"A CRAC mutant that has lost the capacity to bind PI3K products does not support chemotaxis and shows minimal ACA activation"
Phosphoinositide 3-kinase activity controls the chemoattractant-mediated activation and adaptation of adenylyl cyclase.
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CRAC is rapidly recruited to the plasma membrane on chemoattractant stimulation and binds 3-phosphoinositides via its PH domain.
"CRAC is rapidly and transiently recruited to the plasma membrane around the entire periphery of the cell"
Selective membrane exclusion in phagocytic and macropinocytic cups.
Time-resolved responses to chemoattractant, characteristic of the front and tail of Dictyostelium cells.
Locally controlled inhibitory mechanisms are involved in eukaryotic GPCR-mediated chemosensing.
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The PIP3 reporter PHCrac-GFP accumulates at the front of cells in a cAMP gradient, and CRAC contributes to actin regulation during chemotaxis.
"Both CRAC and Akt/PKB play roles in the regulation of actin polymerization during chemotaxis"
High-throughput analysis of spatio-temporal dynamics in Dictyostelium.
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dagA is among the developmentally null mutants identified by high-throughput phenotyping.
"mutants in which genes such as mkpA, piaA, yakA and dagA are disrupted"
Delineating the core regulatory elements crucial for directed cell migration by examining folic-acid-mediated responses.
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The PH domain of CRAC is a biosensor for leading-edge plasma-membrane phosphoinositides, and pseudopod-enriched proteins relocalize to the plasma membrane on uniform stimulation.
"a biosensor that measures increased levels of these plasma membrane (PM) phosphoinositides"
Proteomic Analysis of Heavy Metal-Induced Toxicity Using the Cellular Slime Mould Dictyostelium discoideum: Effects of Copper Exposure on Aggregation and Protein Expression.
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The CRAC protein spot disappeared from 2D gels after copper exposure, correlating copper toxicity with reduced CRAC abundance.
"spot a corresponded to formin-1, spot b corresponded to CRAC"
Chemoattractant and GTP gamma S-mediated stimulation of adenylyl cyclase in Dictyostelium requires translocation of CRAC to membranes.
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Chemoattractant stimulation drives translocation of CRAC from the cytosolic to the membrane fraction, required for adenylyl cyclase activation.
"chemoattractants promote translocation of CRAC from the cytosolic to the membrane fraction"
CRAC, a cytosolic protein containing a pleckstrin homology domain, is required for receptor and G protein-mediated activation of adenylyl cyclase in Dictyostelium.
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dagA is the structural gene for CRAC and is required for receptor and G protein-mediated activation of adenylyl cyclase.
"is required for receptor and G protein-mediated activation of adenylyl cyclase in"
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CRAC is proposed to connect free Gbetagamma subunits to adenylyl cyclase activation.
"CRAC acts to connect free G protein beta gamma subunits to adenylyl cyclase activation"
The Dictyostelium mitogen-activated protein kinase ERK2 is regulated by Ras and cAMP-dependent protein kinase (PKA) and mediates PKA function.
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CRAC is required for proper adaptation of the MAP kinase ERK2.
"CRAC, a PH domain-containing protein required for adenylyl cyclase activation, is also required for proper ERK2 adaptation"
G protein signaling events are activated at the leading edge of chemotactic cells.
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CRAC translocates to inner-face plasma membrane binding sites selectively at the leading edge in a chemoattractant gradient.
"The translocation of the PH domain-containing protein CRAC in D. discoideum to binding sites on the inner face of the plasma membrane"