Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
The subunit composition of the human NADH dehydrogenase obtained by rapid one-step immunopurification.
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Identifies NDUFA10 among the human Complex I polypeptides resolved by immunopurification and mass spectrometry, establishing its complex membership.
"we can resolve and identify the human homologues of 42 polypeptides detected so far"
Accessory subunits are integral for assembly and function of human mitochondrial complex I.
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Systematic knockout of each Complex I accessory subunit shows 25 are strictly required for assembly of a functional complex and that loss of a subunit destabilizes others in the same module, establishing accessory subunits as structural/assembly components.
"25 subunits are strictly required for assembly of a functional complex"
Architecture of Human Mitochondrial Respiratory Megacomplex I(2)III(2)IV(2).
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Cryo-EM of the human respiratory megacomplex resolves the precise assignment of individual human Complex I subunits, including the inner-membrane placement of NDUFA10.
"reveals the precise assignment of individual subunits of human CI"
Assembly of mammalian oxidative phosphorylation complexes I-V and supercomplexes.
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Review of OXPHOS complex assembly; describes supernumerary subunits as playing essential roles in assembly, regulation and stability rather than catalysis.
"play essential roles in assembly, regulation and stability"
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
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Defines a high-confidence human mitochondrial proteome (MitoCoP); source of the high-throughput mitochondrial localization annotation for NDUFA10.
"defined a mitochondrial high-confidence proteome of >1,100 proteins (MitoCoP)"
cDNA of eight nuclear encoded subunits of NADH:ubiquinone oxidoreductase: human complex I cDNA characterization completed.
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Characterizes nuclear-encoded Complex I subunits located in the hydrophobic protein fraction, whose main function is electron transport from NADH to ubiquinone.
"Its main function is the transport of electrons from NADH to ubiquinone"
Complex I oxidises NADH to NAD+, reduces CoQ to CoQH2
Intermediate 1 binds HP subcomplex to form Intermediate 2
Peripheral arm subunits bind the 815kDa complex to form a 980kDa complex
Intermediate 2 binds MT-ND1:NDUFAF5:NDUFAF6 to form a 315kDa subcomplex
The MCIA complex, NDUFAF2-7 all dissociate from the 980kDa complex, resulting in Complex I
ND4, ND5 bind the 550kDa complex to form the 815kDa complex
The 315kDa subcomplex binds the 370kDa subcomplex to form the 550kDa complex