"We have also observed in vivo interactions between Smad2 and MEF2A using co-immunoprecipitation assays."

"The crystal structure of a phosphorylated Smad2, at 1.8 A resolution, reveals the formation of a homotrimer mediated by the C-terminal phosphoserine (pSer) residues."

"we identified TGF-beta-dependent genes that are differentially regulated by Smad2 and Smad3 under regular growth conditions on a genome-wide scale."

"Nodal, acting through ALK7 and Smad2/3, inhibits proliferation and induces apoptosis in human trophoblast cells."

"Our results are consistent with a series of hydrophobic patches on the MH2 that are able to recognize disordered regions of proteins."

"Both complexes are heterotrimers, comprising two phosphorylated R-Smad subunits and one Smad4 subunit, a finding that was corroborated by isothermal titration calorimetry and mutational studies."

"PPM1A dephosphorylates and promotes nuclear export of TGFbeta-activated Smad2/3."

"Formation of the left-right axis involves a symmetry-breaking signal originating in the node or its equivalents, which increases TGF-beta signaling on the left side of the embryo and ultimately leads to asymmetric patterning of the viscera."

"cholesterol treatment suppresses or attenuates TGF-beta responsiveness in all cell types studied as determined by measuring TGF-beta-induced Smad2 phosphorylation and nuclear translocation, TGF-beta-induced PAI-1 expression, TGF-beta-induced luciferase reporter gene expression and TGF-beta-induced growth inhibition."

"whereas TGF-β increased binding to pri-miR-21 by SMAD2 and SMAD3, but not by SMAD1, indicating that the association between R-SMADs and pri-miR-21 is specifically regulated by ligand stimulation"

"(S/T)P motif phosphorylation also enhanced the interaction of Smad2/3 with Smurf2."

"The IL-8 and E-selectin transcriptional responses were shown to be Smad2-dependent, a novel finding."

"We identified a new motif, termed SMAD complex-associated (SCA), that is bound by SMAD2/3/4 and FOXH1 in human embryonic stem cells (hESCs) and derived endoderm."

"BMP-3b suppressed the expression of osteoblastic markers including Runx2, osteocalcin and type-1 collagen induced by BMP-2, -4, -6 and -7."

"Pin1 does not only affect Smad phosphorylation and stability but also regulates subcellular localization of Smad2 and supports its binding to phosphorylated tau protein."

"The SMAD2 MH1 domain and full-length proteins also bound to different 5GC probes (Gsc1 and Gsc2 probes) from the Gsc promoter in the 0.6–1.2 µM concentration range"

"the full-length SMAD4 protein bound the Gsc1 probe at values between 0.15 and 0.30 µM (Fig."

"Thus activated, SMAD2 and SMAD3 form complexes with SMAD4, accumulate in the nucleus, and recruit coactivators and repressors to regulate the expression of target genes."

"TGF-β, Nodal, and Activin receptors phosphorylate SMAD2 and SMAD3."

"We observe that properly folded SMAD2 protein has intrinsic DNA-binding activity, which is modulated by the ensemble of conformations adopted by the E3 insert in solution."

"the stimulatory effect of GDF-8 on MMP2 expression is mediated by the ALK5-SMAD2/3 signaling pathway."

"MADR2 and not the related protein DPC4 transiently interacts with the TGFbeta receptor and is directly phosphorylated by the complex on C-terminal serines."

"Smad6 interacted with all species tested (Smad1, Smad2, and Smad4), whereas Smad7 interacted only with Smad6 (Fig."

"Smads 2, 3 and 4 accumulated in the nucleus upon TGF-beta1 treatment in Mv1Lu cells, and showed a synergistic effect in a transcriptional reporter assay using the TGF-beta-inducible plasminogen activator inhibitor-1 promoter."

"Smads 2 and 3 interacted with Smad4 after TbetaR activation in transfected COS cells."

"we observed TbetaR-activation-dependent interaction between Smad2 and Smad3."

"Overexpression of Smad2 has the same effect [Fig."

"hFAST-1 shared with xFAST-1 the ability to bind to human Smad2 and activate an activin response element (ARE)."

"The hFAST-1-dependent activation of ARE was completely dependent on endogenous Smad4 and stimulation by a TGF beta-like ligand."

"Based on the observed patterns of expression, we speculate that individual or combinations of Smads may play specific roles in cell-fate determination during kidney development."

"selective removal of both Smad2 and Smad3 from the epiblast additionally disrupts specification of axial and paraxial mesodermal derivatives."

"graded Nodal/Smad2 signals govern allocation of the axial mesendoderm precursors that selectively give rise to the ADE and PCP mesoderm."

"faulty versican clearance due to ADAMTS5 deficiency blocks the initiation of pSmad2 signaling, which is required for excavation of endocardial cushions during aortic and pulmonary valve development."

"Mainly localized to the nucleoplasm and nucleoli."

"Our paper is the first to show that the Smad2,4/LEF1 complex replaces beta-catenin/LEF1 during activation of EMT in vivo by TGFbeta3."

"IPO7 could bind to P-SMAD2 and affect the nuclear-cytoplasm-shuttling of P-SMAD2."

"Hetero-oligomerization of Smad4 with the pathway-restricted SMAD proteins is essential for Smad4-mediated transcription."

"We found that Smad2, but not Smad3, is required to maintain the undifferentiated pluripotent state."

"Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 or with IPO7"