Gene Ontology annotation through association of InterPro records with GO terms

Combined Automated Annotation using Multiple IEA Methods

Pseudomonas putida KT2440 strain metabolizes glucose through a cycle formed by enzymes of the Entner-Doudoroff, Embden-Meyerhof-Parnas, and pentose phosphate pathways

In KT2440 cell-free extracts, isocitrate dehydrogenase (Icd/PP_4011) shows ~89% activity with NADP+ versus ~11% with NAD+, confirming NADP+ specificity and a role in NADPH supply.

PMID:30936206

Multi-omics analysis unravels a segregated metabolic flux network that tunes co-utilization of sugar and aromatic carbons in Pseudomonas putida

Phosphorylation of the ICD-associated protein PP_4011 changes with carbon source, consistent with post-translational tuning of flux at the TCA/glyoxylate branch point.