PA0949 (Q9I509) review notes

WrbA-family flavodoxin-like NAD(P)H:quinone oxidoreductase of P. aeruginosa PAO1.
No gene name in UniProt; folder and gene_symbol use the locus tag.

Literature (no deep-research provider available; own reading)

Green et al. 2014 (abstract only, no PMC): five P. aeruginosa quinone
reductases, including WrbA, purified and assayed. PMID:25085734 Over-expression gave no H2O2 protection in wild type
PMID:25085734, only in a katA
mutant or in P. putida.
Patridge & Ferry 2006 (full text, PMC1482846): E. coli and A. fulgidus WrbA are
NQOs; WrbA family = type IV NQO. P. aeruginosa appears only as a solved
structure PMID:16672604. The PseudoCAP IDA of GO:0016655 to
PA0949 citing this paper is therefore a wrong-reference row (CONFIRMED).
Gorman & Shapiro 2005 (full text, PMC2253248): crystal structures of P.
aeruginosa WrbA with FMN, homotetramer PMID:16322580.
PubMed MCP search returned API errors on 2026-11-05; PMC id conversion showed no
full text for PMID:25085734.

Seed support queried live in QuickGO on 2026-11-05 (seed MOD ids mapped to UniProt).

PTN node	node term	n_seeds	leaf IBAs	seed annotation (term, evidence, PMID)	verdict	rationale	other seeds	IBD stands without this seed?
PTN000777387	GO:0003955 NAD(P)H dehydrogenase (quinone) activity	4	363 (76 taxa)	GO:0003955, IMP, PMID:25085734 (PseudoCAP)	SOUND	Abstract states the purified PA enzyme reduces quinones in vitro; the term is right, the code would better be IDA.	E. coli WrbA P0A8G6 (IDA/IMP PMID:16672604), yeast PST2 Q12335 (IDA PMID:28499769), Arabidopsis FQR1 Q9LSQ5 (IDA PMID:11842161)	Yes; three independent IDA seeds across bacteria, fungi and plants

No PSEUDOCAP or GO_CENTRAL defect for the seeding row. Node term suits the WrbA clade.

Other findings on this gene:

GO:0016655 IDA (PseudoCAP, PMID:16672604): wrong reference, PSEUDOCAP defect
recorded (CONFIRMED, full text read). This row does not seed an IBD.
GO:0016020 membrane IBA at the same node: seeded only by fungal Pst/Ycp4/Rfs1
proteins (PM / membrane raft). Marked over-annotated for PA0949; no defect filed
(PA0949 is not a seed for it), raised as a question.
GO:0034599 IMP: over-expression in a katA mutant only; marked over-annotated.