Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Receptor-mediated endocytosis in the Caenorhabditis elegans oocyte.
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Beta-adaptin RNAi abolishes yolk uptake and causes embryonic defects like clathrin and alpha-adaptin RNAi.
"elegans clathrin heavy chain, α-adaptin, and β-adaptin RNAi all produced similar defects in oocytes and embryos."
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Beta-adaptin links AP-2 to clathrin.
"The β-adaptin subunit is thought to connect the AP2 complex to clathrin ( Pearse and Robinson, 1990 )."
Clathrin and AP2 are required for phagocytic receptor-mediated apoptotic cell clearance in Caenorhabditis elegans.
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apb-1 RNAi causes persistent, un-internalised germ-cell corpses with defective actin recruitment and phagosome maturation.
"Similarly, 12 of 25 corpses (48%) from 7 gonad arms of apb-1(RNAi) animals were found not to be internalized (Figure 1D)."
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APB-1 binds CED-6 directly.
"GST-CED-6 directly interacted with 35S-labeled APA-2, APB-1 and DPY-23, and His6-tagged CHC-1C (Figure 4A)."
AP2 hemicomplexes contribute independently to synaptic vesicle endocytosis.
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The worm beta-adaptin is shared by AP-1 and AP-2 and pairs with mu2 as a stable hemicomplex.
"Here we demonstrate that in the absence of α-adaptin that a μ2-β hemicomplex remains, and that in the absence of μ2-adaptin that a α-σ2 complex remains in vivo."
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APB-1::GFP localises to nerve-ring synapses and the oocyte plasma membrane.
"Tagged β-adaptin and μ2-adaptin are localized to synaptic regions of the nerve ring (the major neuropil of the worm, Figure 6B,C) and at the plasma membrane in oocytes (Figure 6—figure supplement 1)."
Mu2 adaptin facilitates but is not essential for synaptic vesicle recycling in Caenorhabditis elegans.
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beta2 adaptin RNAi abolishes yolk uptake.
"RNA interference against clathrin, α adaptin, or β2 adaptin each abolished uptake of a GFP-tagged vitellogenin (YP170-GFP) into oocytes."
Deep research report (falcon/Edison) on C. elegans apb-1
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APB-1 is the beta subunit shared by AP-1 and AP-2 and localises to intestinal TGN puncta with SMAP-1 (Wang et al. 2021).
"GFP–APB-1 labels punctate structures and colocalizes with SMAP-1, an Arf-family GAP implicated in TGN coat assembly and polarized secretion."