GO_REF:0000044

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

file:PSEPK/algI/algI-uniprot.txt

UniProtKB reviewed entry for algI (Q88ND2)

The record identifies a multi-pass membrane-bound acyltransferase-family component of alginate O-acetylation.

file:PSEPK/algI/algI-goa.tsv

QuickGO GOA annotations for algI

The fetched GOA table contains the three annotations reviewed here.

file:interpro/panther/PTHR13285/PTHR13285-metadata.yaml

PANTHER acyltransferase family metadata containing AlgI

The PANTHER SF23 teichoic-acid D-alanyltransferase label is not used for the alginate-specific assignment.

PMID:12003941

Mutant analysis and cellular localization of the AlgI, AlgJ, and AlgF proteins required for O acetylation of alginate in Pseudomonas aeruginosa.

Nonpolar algI deletion abolishes alginate O-acetylation, complementation restores it, and topology mapping identifies seven transmembrane segments.

PMID:8636017

Identification of algI and algJ in the Pseudomonas aeruginosa alginate biosynthetic gene cluster which are required for alginate O acetylation.

Genetic analysis separates the algIJF acetylation requirement from polymer biosynthesis and C5 epimerization.

PMID:31900562

Analysis of the alginate O-acetylation machinery in Pseudomonas aeruginosa.

AlgI participates in the AlgI-AlgJ-AlgF complex and its stability is coupled to AlgF and Alg8.

file:projects/P_PUTIDA/deep-research/PSEPK__alginate-o-acetylation__ppu00543-deep-research-openscientist.md

OpenScientist PSEPK alginate O-acetylation synthesis

The report identifies AlgI as the inner-membrane acetyl-transfer component of the complete AlgI/J/F/X machinery.

file:modules/alginate_o_acetylation.yaml

Alginate O-acetylation module

AlgI implements the membrane acetyl-donor transfer part.