Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on Enzyme Commission mapping
Electronic Gene Ontology annotations created by ARBA machine learning models
UniProtKB entry A0A0H2MDH4 (A0A0H2MDH4_VARPD): Methanol dehydrogenase [cytochrome c] subunit 1
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A0A0H2MDH4 (VPARA_00790) is a 608-residue periplasmic PQQ-dependent alcohol/methanol dehydrogenase (EC 1.1.2.7; NCBIfam TIGR03075, InterPro IPR017512, CDD cd10278) with a cleavable N-terminal signal peptide (residues 1-28) and mature chain (residues 29-608) lacking transmembrane helices.
"FT SIGNAL 1..28
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 29..608
FT /evidence="ECO:0000256|SAM:SignalP""
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Binds one pyrroloquinoline quinone (PQQ) prosthetic group per subunit via conserved active-site residues (positions 83, 136, 180, and 266) and contains the proton-acceptor active site Asp328.
"CC -!- COFACTOR:
CC Name=pyrroloquinoline quinone; Xref=ChEBI:CHEBI:58442;
CC Evidence={ECO:0000256|PIRSR:PIRSR617512-2};
CC Note=Binds 1 PQQ group per subunit. {ECO:0000256|PIRSR:PIRSR617512-2};"
PQQ-dependent methanol dehydrogenases: rare-earth elements make a difference.
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Establishes that Gram-negative PQQ-dependent methanol and alcohol dehydrogenases are periplasmic eight-bladed beta-propeller quinoproteins that coordinate either Ca(2+) (MxaFI-type) or a trivalent rare-earth element (REE/lanthanide, XoxF-type) in the active site, with REE coordination requiring a specific additional aspartate residue near the catalytic site.
"While structures of MxaFI- and XoxF-MDH are conserved, also regarding the binding of PQQ, the accommodation of a REE requires the presence of a specific aspartate residue near the catalytic site."