Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on Enzyme Commission mapping
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Combined Automated Annotation using Multiple IEA Methods
Characterizing fatty acid oxidation genes in Drosophila.
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CRISPR-Cas9 loss-of-function of Arc42, the higher-scoring of two predicted Drosophila ACADS orthologs, produces elevated C4 (butyrylcarnitine) that is exacerbated by starvation, recapitulating the acylcarnitine signature of human and mouse SCAD deficiency; the paralog CG4860 does not, identifying Arc42 as the functional fly short-chain acyl-CoA dehydrogenase.
"Arc42 knockout flies exhibited the C4 elevation characteristic of humans and mice lacking functional SCAD, while CG4860 knockout flies did not"