The architecture of EMC reveals a path for membrane protein insertion.

Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity

GO_REF:0000033

Annotation inferences using phylogenetic trees

GO_REF:0000044

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

GO_REF:0000107

Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara

GO_REF:0000108

Automatic assignment of GO terms using logical inference, based on on inter-ontology links

GO_REF:0000117

Electronic Gene Ontology annotations created by ARBA machine learning models

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

PMID:19946888

Defining the membrane proteome of NK cells.

PMID:22119785

Defining human ERAD networks through an integrative mapping strategy.

Affinity-MS ERAD-network mapping that first identified the EMC (including MMGT1/EMC5) in human cells and localized it to the ER membrane.

PMID:26496610

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.

PMID:28514442

Architecture of the human interactome defines protein communities and disease networks.

PMID:29242231

The ER membrane protein complex is a transmembrane domain insertase.

EMC is a transmembrane domain insertase; depletion of EMC5/EMC6 reduces client insertion.

PMID:29809151

The ER membrane protein complex interacts cotranslationally to enable biogenesis of multipass membrane proteins.

The EMC engages multipass membrane protein clients cotranslationally to enable their biogenesis.

PMID:30415835

EMC Is Required to Initiate Accurate Membrane Protein Topogenesis.

The EMC sets the N-exo topology of the first TMD of GPCRs and other multipass proteins.

PMID:32296183

A reference map of the human binary protein interactome.

PMID:32439656

Structural basis for membrane insertion by the human ER membrane protein complex.

Cryo-EM structure of the human EMC; defines MMGT1/EMC5 topology and intra-complex contacts.

PMID:33961781

Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.

PMID:34918864

EMC is required for biogenesis of Xport-A, an essential chaperone of Rhodopsin-1 and the TRP channel.

In vivo Drosophila evidence that the EMC, including EMC5, is required for TMD membrane insertion of a tail-anchored client.

PMID:35271311

OpenCell: Endogenous tagging for the cartography of human cellular organization.

PMID:40355756

The solute carrier superfamily interactome.

PMID:37199759

A selectivity filter in the ER membrane protein complex limits protein misinsertion at the ER.

The EMC hydrophilic vestibule acts as a charge-based selectivity filter that rejects mitochondrial tail-anchored proteins and enforces the positive-inside rule for multipass substrates; EMC5/MMGT1 is a core membrane subunit of this insertase/selectivity machine.

PMID:37196677

EMC chaperone-Ca(V) structure reveals an ion channel assembly intermediate.

Cryo-EM of an EMC-bound CaV1.2 assembly intermediate shows the EMC functions as a holdase/chaperone during multipass channel assembly, extending EMC function beyond insertion; EMC5 is a constitutive core subunit of this machine.

PMID:37957425

EMC rectifies the topology of multipass membrane proteins.

C-terminal TMDs of mammalian multipass proteins are inserted post-translationally by the EMC to rectify topology and complete biogenesis; this sequential co-/post-translational mechanism may apply to ~250 diverse multipass proteins. EMC5 is a core subunit of the EMC.

PMID:38517390

Structural insights into human EMC and its interaction with VDAC.

Cryo-EM structures of human EMC in apo and VDAC-bound states reveal a gating plug in the hydrophilic vestibule and an EMC-VDAC interaction at mitochondria-ER contact sites; endogenous human EMC was purified via a Twin-Strep tag on EMC5, showing EMC5 is a tractable handle for native EMC.

PMID:40753078

The EMC acts as a chaperone for membrane proteins.

Beyond TMD insertase activity, the EMC has a chaperone function engaging TMDs via its EMC1 subunit and modulating their orientation in the bilayer; productive TMD assembly reduces binding to the chaperone site. EMC5 is a core EMC subunit.

PMID:37269834

Identification of host regulators of Mycobacterium tuberculosis phenotypes uncovers a role for the MMGT1-GPR156 lipid droplet axis in persistence.

A genome-wide CRISPR screen prioritized MMGT1; MMGT1-deficient macrophages promote a switch of M. tuberculosis toward persistence, with upregulated lipid metabolism and lipid droplet accumulation driven by the orphan GPCR GPR156, and triacylglycerol-synthesis inhibition reduces both droplets and persistence.