Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on Enzyme Commission mapping
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Mapping organelle proteins and protein complexes in Drosophila melanogaster.
An inventory of peroxisomal proteins and pathways in Drosophila melanogaster.
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Computational peroxisomal-proteome inventory (targeting-signal prediction) that is the source of both the ISM mitochondrion and ISM peroxisome predictions for Acat1. Only five predicted peroxisomal proteins were experimentally confirmed, and the abstract does not indicate whether Acat1 was among them.
OpenScientist audit of the Acat1 peroxisome annotation
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Focused audit of the Faust et al. peroxisomal-proteome prediction and live QuickGO provenance found that Acat1 was not among the Drosophila proteins experimentally confirmed as peroxisomal.
"**Acat1 (Q9W3N9) is not among them.**"
Glia fuel neurons with locally synthesized ketone bodies to sustain memory under starvation.
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Identifies CG10932 as the Drosophila ortholog of human mitochondrial acetoacetyl-CoA thiolase ACAT1 (DIOPT 15/15) and shows by RNAi that Acat1 is required in adult mushroom body neurons for ketone-body oxidation, generating acetyl-CoA in mitochondria to sustain memory under starvation.
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Describes ketogenesis in cortex glia as proceeding via a thiolase, HMGS and HMG-CoA lyase; the thiolase step of ketogenesis is stated generically rather than assigned to CG10932, whereas the gene experimentally assayed as ACAT1 is the neuronal ketone-body-oxidation enzyme.
FlyBase gene description for Acat1 (DROME)