Tes (testin), Rattus norvegicus (UniProtKB:Q2LAP6) - manual literature synthesis

No deep-research provider API keys were available in this session, so this synthesis was written by
the reviewer from cached publications (publications/PMID_*.md) and PubMed searches (2026-10-28).
Full text was available for PMID:21278383 and PMID:12695497; the others were read as abstracts only.

Identity and domain architecture

• Rat Tes (Q2LAP6, 419 aa, RGD:1566346, GeneID 500040) is the orthologue of human TES (Q9UGI8) and
  mouse Tes (P47226). It has an N-terminal PET domain (residues 92-199) and three C-terminal LIM
  zinc-binding domains (232-295, 297-357, 360-419) [UniProtKB:Q2LAP6 FT DOMAIN], and "Belongs to the
  prickle / espinas / testin family" [UniProtKB:Q2LAP6].
• The N- and C-terminal halves bind each other, a closed conformation that masks the zyxin site
  PMID:12695497.

Localisation

• Human TES localises to focal adhesions, stress fibres and cell-cell contacts
  PMID:12571287.
• Recruitment to focal adhesions depends on zyxin, which binds LIM1
  PMID:12695497;
  PMID:21278383.
• In rodent testis, Tes is in the subacrosomal layer (acroplaxome) of round spermatids together with
  Arp7A (ACTL7A) and Mena, but not on the Golgi PMID:21278383. GO has no acroplaxome term.

Interactions (binding activity)

• Rat-specific: Tes co-immunoprecipitates with Arp7A and Mena from rat testis
  PMID:21278383.
• LIM2(-3) binds the N-terminal extension of Arp7A directly (crystal structure of
  1-65Arp7A-LIM2-3Tes-EVH1Mena) PMID:21278383.
• LIM3 binds the EVH1 domain of Mena, but not VASP or EVL, occluding the FPPPP site
  PMID:18158903.
• Tes is in complexes with actin, Mena and VASP in cells
  PMID:12695497;
  chicken TES pulls down zyxin and actin PMID:14743347.
• Zinc: the LIM2-3 crystal structure contains two zinc ions per LIM domain
  PMID:21278383.

Cellular roles (phenotypes, human/chicken cells)

• Overexpression increases cell spreading on fibronectin PMID:12571287; chicken TES overexpression
  increases spreading and decreases motility PMID:14743347.
• RNAi loss of TES in HeLa cells causes loss of stress fibres and reduced RhoA activity
  PMID:15662727.
• LIM3 regulates Mena-dependent migration PMID:18158903.
• Growth suppression / candidate tumour suppressor: forced expression reduces colony formation
  PMID:11420696;
  negatively regulates T47D proliferation PMID:12695497. These are overexpression phenotypes in tumour
  lines; a direct mechanism is not established.

No rat-specific functional studies beyond PMID:21278383 were found.