ECP6 (Fulvia fulva / Cladosporium fulvum; UniProt B3VBK9) - curation notes

Sources

• UniProt B3VBK9 record (ECP6-uniprot.txt); 3 LysM domains, PDB 4B8V/4B9H.
• ECP6-deep-research-falcon.md (Falcon/Edison deep research).
• PMID:18452583 Bolton et al. 2008 (abstract-only cache) - discovery, apoplastic secretion, virulence.
• PMID:20724636 de Jonge et al. 2010 Science (abstract-only cache; PubMed metadata verified) - chitin sequestration, PTI suppression.
• PMID:23840930 Sanchez-Vallet et al. 2013 eLife (full text cached) - crystal structure, intrachain LysM1-LysM3 groove.

Identity / localization

• Secreted into tomato leaf apoplast during infection PMID:18452583; Ecp6 identified among proteins secreted during C. fulvum-tomato interactions
  PMID:18452583.
• Virulence factor: RNAi silencing reduces virulence; heterologous expression in Fusarium oxysporum increases virulence
  PMID:18452583.

Molecular function: chitin binding

• PMID:20724636
• Structure: intrachain LysM1-LysM3 dimerization forms buried groove; ultra-high affinity
  PMID:23840930
  PMID:23840930.
• LysM2 binds chitin with low micromolar affinity yet still suppresses chitin-triggered immunity, proposed via receptor-complex perturbation (hypothesis)
  PMID:23840930.
• Glycan array / chitosan / cellulose specificity and failure to protect hyphae against chitinases (in contrast to Avr4) are reported in de Jonge 2010 full text
  (not cached; abstract only); taken from deep research and UniProt FUNCTION text. Not quoted in YAML.

Biological process

• PMID:20724636
• GO:0140423 effector-mediated suppression of host pattern-triggered immunity signaling (EXP, PMID:23840930) - correct symbiont-side term; matches
  the term used for M. oryzae Slp1 (genes/PYRO7/slp1).

Decisions

• All 4 GOA rows ACCEPT (2x extracellular region, chitin binding, GO:0140423).
• No NEW terms: no chitinase-protection term (negative evidence); no receptor/perception terms (those belong on CEBiP/CERK1);
  a host-apoplast-specific CC term was considered but not added - extracellular region is adequate and consistent with slp1.
• Project question 2 (LysM effector vs receptor leakage): no InterPro2GO rows on ECP6 at all; effector carries only the activity term
  (chitin binding) and the symbiont-side process term, no "chitin-mediated signaling"/"defense response" leakage.