ENO1 (P00924) notes

Activity and isozymes

• Enolase (EC 4.2.1.11), Mg2+-dependent [UniProt:P00924 "Reaction=(2R)-2-phosphoglycerate = phosphoenolpyruvate + H2O;"].
• Two enolase genes, ENO1 and ENO2; polypeptides assort randomly into three active dimers PMID:6282834.
• Carbon-source regulation PMID:6282834.
• ENO1 non-essential, ENO2 essential PMID:16565073.
• Reversible reaction -> glycolysis and gluconeogenesis (YeastPathways RCA for both is reasonable).

Moonlighting / minor locations

• Vacuole fusion stimulation independent of catalysis PMID:16565073; small vacuole-bound pool PMID:16565073.
• Mitochondrial-surface complex PMID:16962558.
• Cell surface (Eno2 annotation) PMID:16544286, noted as controversial by the authors.
• Melatonin affinity capture PMID:31708896: abundance-biased pulldown; weak evidence for real melatonin binding.

Pathway-context notes

• GLUCFERMEN-PWY RCA -> "bifid shunt" is a mapping artefact (Bifidobacterium pathway); removed.
• GO:0061620 obsolete; replaced by glycolysis.