Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on Enzyme Commission mapping
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Characterization of the osmoprotectant transporter OpuC from Pseudomonas syringae and demonstration that cystathionine-beta-synthase domains are required for its osmoregulatory function.
-
The P. syringae DC3000 OpuC transporter (ATPase Q87WH5, PSPTO_4575) is a high-affinity glycine betaine and low-affinity choline osmoprotectant importer with broad QAC specificity, and its ATPase CBS domains are required for function.
"The P. syringae pv. tomato OpuC transporter had a high affinity for glycine betaine, a low affinity for choline, and a broad substrate specificity that included acetylcholine, carnitine, and proline betaine."
-
The only PA3891 experiment is that a PAO1 genomic clone carrying PA3891 restored growth of a DC3000 opuCA-deficient triple mutant under hyperosmotic conditions with betaine or choline.
"The sequence of the complementing clones indicated that transporters involving Psyr4249 from P. syringae pv. syringae strain B728a and PA3891 from P. aeruginosa PAO1 were functional, as predicted."
The ATP-binding cassette transporter Cbc (choline/betaine/carnitine) recruits multiple substrate-binding proteins with strong specificity for distinct quaternary ammonium compounds.
Roles of three transporters, CbcXWV, BetT1, and BetT3, in Pseudomonas aeruginosa choline uptake for catabolism.