ELOVL4 source evidence and access guide

The 37 retained source contexts cover every one of the 128 current raw QuickGO records and the genuine 128-row normal TSV. The grouped HuRI source retains all 92 imported partners. These different row counts describe representation, not missing assertions. All 32 inherited excerpt occurrences are preserved; no new publication quotations are added.

The sole official natural product is Q9GZR5, 314 aa. Tagged human cDNAs, truncations, rat W246G and conditional mouse alleles are experimental contexts, not equivalent natural products. A normal cache flag describes the cached text; the assessed original scope is stated separately below.

Reference assessments

Operative original locators

• PMID 11138005: DOI 10.1038/83817, recovered original indexed Methods/Results/Discussion, operative line windows 200–256, 502–542, 886–1033, 1102–1216, 1218–1278 and 1339–1446. No figure pixels or supplementary material.
• PMID 16036915: DOI 10.1074/jbc.M503411200, original cloning/expression and localization/complex Methods, ER-localization Figure 4 and coexpression-versus-mixed-extract results. Actual original captions were inspected; no pixels or supplementary material.
• PMID 20937905: PMC2973002, Figure 1B and indexed main Results record 24; the CERS2/ER observations are stated in the main text with S8B/S2 references. Main HTML SHA256 cf1a6682131e8ffb27129a81b5bde789f6b66e1e0e45b6e631878ab3a0b1cbc4. Figure 1 pixels were independently inspected; SI was not recovered.
• PMID 23479632: complete cached scientific paragraphs 13–57, especially 18, 25, 27–29, 40–42, 44, 47, 49 and 53–57. Mouse conditional-loss and human disease-transgene experiments remain separate.
• PMID 32296183: selected cached paragraphs 12, 13, 16, 17, 19, 44–45 and legends 50/54. The portable IntAct proof joins every imported partner to literal records without converting three method records into independent biological replications.
• PMID 24736612: PMC3988159, original indexed blocks 6–62; operative blocks 7, 9, 17, 21, 38, 42, 48 and 60. XML SHA256 32972e1aaaf5a60ecfc1ad26bdbcbe746238de2db3dc2bb7887071e9942bdb9e.
• PMID 32780351: PMC7515967, original indexed blocks 8–31 and 34–71; operative blocks 9, 11, 13, 14, 16, 39, 41, 51, 52, 63, 64, 67 and 70. XML SHA256 43bb854e75c2aa82bd7767965f782cdcf1dee52477dec4e5a3fc756866e2f38c.
• PMID 37491316: PMC10436685, original indexed blocks 8–52; operative blocks 10, 12, 13, 22, 24, 27, 33, 35, 47 and 49–52. HTML SHA256 71f492df3dfa2668998da23aa6531dc4e1ee9fd3a4f8438d1fade0c9d87ee4cb. Results, rather than the inconsistent Discussion phrase about inhibitory frequency, anchor the directional assessment.

Database provenance and specificity

The portable analysis reconstructs the complete PTHR11157 target path and literal IBD records. The ungapped target sequence exactly matches the official 314-aa product. All seven inherited placements are valid; current donor-list differences are shown rather than silently harmonized. Current complete rat, mouse and human ELOVL1 annotations are distinct from the ancestral placement itself.

ARBA00028259 and UR000254926 have satisfied target condition sets. ARBA00028685 retains 39 unresolved sets because FunFam assignments were not captured; ARBA00044377 returned HTTP 404. Neither gap disproves the independently supported ER location or catalytic activity. The historical UniPathway route was not recovered.

Rows 9 and 16 are explicitly lateral term changes. GO:0019368 unsaturated fatty acid elongation and GO:0006636 unsaturated fatty acid biosynthetic process do not contain one another through the captured is_a paths. GO:0042761 very long-chain fatty acid biosynthetic process and GO:0035338 long-chain fatty-acyl-CoA biosynthetic process are also lateral; the latter definition covers acyl groups of 13–22 carbons, whereas the former covers fatty acids longer than 22 carbons.

Source-index decision map

Cache-state access flags

The optional full_text_unavailable flags follow the normal-cache convention for PMIDs 11138005, 16036915, 20937905, 37491316 and 38422897. The first four have separately recovered original material with the precise read scopes recorded above; their flags do not negate that access. Only 38422897 still lacks operative ELOVL4-specific evidence. No normal publication cache was edited to reconcile these distinct states.

Follow-up interpretation and reproducibility boundaries

The current annotations retain the same decisions and source fields. In particular, the circadian row is scientifically unresolved despite complete original access, and the two positive generic-binding sources remain non-core under the campaign policy. The seven IBA support lists link to the reproducible PAINT results.

The core's specific processes are saturated fatty-acid elongation and polyunsaturated fatty-acid elongation, replacing their broad elongation ancestor. Official typed is_a paths place both below GO:0030497; neither contains the other or GO:0042761, and GO:0042761 contains neither. The greater-than-22-carbon biosynthesis term therefore retains complementary chain-length information. Human ELOVL4 membrane assays support saturated substrates, while accepted PAINT evidence and endogenous mouse conditional loss support the polyunsaturated role; this does not assign a precise purified-human PUFA product distribution.

VERIFIED reference assessments concern the identified source and the stated claim, not unrestricted local availability of every experiment. The normal abstract-only caches for 11138005, 16036915, 20937905 and 37491316 remain unchanged; separately recovered original text is available only through the cited external locators and preserved private access records. For 16036915, Figure 4 refers to read text/captions, not inspected pixels. For 20937905, Figure 1 pixels were inspected, but S2 and S8B were read only as observations reported in the main Results; their detailed Methods and images remain unavailable. The portable archive contains database inputs, not article bodies, and its unchanged outputs verify those database derivations rather than publication interpretation.

2026-10-23 feedback clarification

The annotation support lists now cite this guide for the HuRI and CERS2 associations, the unresolved TECR source, and the short-term potentiation transfer. These are provenance links; they add no publication quotations or new annotations. All 37 actions remain unchanged.

For the affected positive claims, the original locators are:

• PMID 37491316 original article: Methods, Animals and Electrophysiology; Results on parallel-fiber to Purkinje-cell short-term plasticity, Figure 3A for paired-pulse facilitation at 20, 50 and 100 ms, Figure 3B,D for stimulus trains, and Figure 3E for kynurenic-acid sensitivity. The Discussion paragraphs on tissue-specific lipid synthesis and possible vesicle-cycle effects distinguish the earlier mouse lipid-supplementation rescue from rescue proposed for W246G rats. Original indexed blocks 10, 12–13, 32–36 and 50–51 identify these passages. Figure 2 and blocks 26–31 provide the different climbing-fiber context. These locators identify read text and captions, not newly inspected figure pixels.
• PMID 20937905 original article: Results section on CERS2 regulation of C24-CoA synthesis, final co-immunoprecipitation paragraph reporting S8B (indexed main block 53). This states association with all seven ELOVLs and explicitly allows indirect association through the elongation machinery. The S8B panel and its detailed supplementary Methods remain unavailable; Figure 4D assays ELOVL1 and is not substituted as an ELOVL4 experiment.
• PMID 32296183 original article: Results section on generation and characterization of HuRI, the paragraph on quadruplicate pairwise retesting and sequence confirmation, with Figure 1c and Extended Data Figure 1g,h for dataset-level orthogonal benchmarking. The separately preserved IntAct records establish the imported ELOVL4 pairs. The main paper's benchmarking does not imply pair-specific MAPPIT or GPCA confirmation for every ELOVL4 interaction.

GO:1990926 is a subtype of regulation of synaptic plasticity, GO:0048167, under modulation of chemical synaptic transmission, GO:0050804. The measured facilitation phenotype supports the retained non-core role. The annotation does not require ELOVL4 to be the neurotransmitter-release machinery or its catalytic rate to change within milliseconds. Lipid-mediated regulation is biologically grounded, while the precise link between the selective W246G lipid defect and this synaptic phenotype remains an inference. That mechanistic limit does not invalidate the observed process phenotype or warrant reversing a sign.

The two supported generic-binding rows remain KEEP_AS_NON_CORE under the explicit campaign instruction to retain supported interactions without inventing a specific molecular function. The unresolved TECR row remains UNDECIDED. A generic-term validator advisory does not negate the supporting experimental association; no main-repository policy change is proposed here.