PET100 curation notes

IBA / PAINT

• The pinned GOA snapshot has three GO_REF:0000033 rows, all with
  PANTHER:PTN002143768|SGD:S000002486 in WITH/FROM.
• Current local PAINT for PTHR33968 places four IBDs on PTN002143768:
  GO:0005743 mitochondrial inner membrane, GO:0044183 protein folding
  chaperone, GO:0033617 mitochondrial respiratory chain complex IV assembly,
  and GO:0051131 chaperone-mediated protein complex assembly.
• The GO:0005743 and GO:0033617 IBAs are retained as NO_FAILURE_CORE.
  They are placed at the PET100-family PTN and match direct yeast evidence.
• The pinned GO:0051082 unfolded protein binding IBA is stale relative to
  current PAINT, which now uses GO:0044183 at the same node. The human PET100
  review marks that replacement MF as over-annotated because PET100 family
  proteins are COX-specific assembly factors, not demonstrated general
  foldases. The unfolded-protein-binding project likewise lists yeast PET100 as
  an over-annotated COX assembly factor. The same reasoning applies here: keep
  the stale row as over-annotated rather than rewriting it to another generic
  chaperone MF.
• GO:0051131 chaperone-mediated protein complex assembly is now an IBD at the
  same PAINT node, seeded by yeast Pet100p, and should appear as an IBA after a
  GOA refresh. I left it out of proposed_new_terms because the retained
  GO:0033617 IBA already captures the specific respiratory-chain complex IV
  assembly process.
• The direct GO:0051082 row is over-annotated for the same biological reason.

Cached literature

• The three older PMID caches used by the review are abstract-only. PMID:38612624
  is a full-text review used only as recent background.
• PMID:11498004 reports Pet100 topology in the inner mitochondrial membrane and
  describes the C-terminal domain as essential for its function as a COX-specific
  assembly facilitator.
• PMID:15507444 places Pet100 in an inner-membrane subassembly with Cox7, Cox8
  and Cox9, absent from mature holoenzyme, supporting a late complex-IV assembly
  role instead of a generic unfolded-client binding activity.
• PMID:16823961 is high-throughput mitochondrial proteomics support for a broad
  mitochondrion location only.

2024-2026 literature check

• PubMed searches for recent PET100/Pet100 papers found no new yeast primary
  studies that change the annotation. The 2024 yeast-to-human COX-deficiency
  review keeps PET100 in late complex IV biogenesis: PMID:38612624.