leu3 (SPBC3E7.16c, UniProt O59736) notes

• 2-isopropylmalate synthase (EC 2.3.3.13). Functional counterpart of S. cerevisiae LEU4/LEU9 (NOT of S. cerevisiae LEU3, which is the Zn-cluster transcription factor). PMID:35325114; PMID:35325114.
• Genetics (full text): PMID:20657649; Phycomyces leuA partially rescues PMID:20657649.
• Leucine feedback: PMID:35325114.
• Localisation: PMID:35325114; UniProt annotates no transit peptide; GO-CAM uses GO:0005739 (mitochondrion), GOA also has IC matrix.
• Implication: with a single mitochondrial IPMS and no known cytosolic form, alpha-IPM export by oac1 may be essential in S. pombe (differs from S. cerevisiae, where cytosolic short Leu4 bypasses Oac1 PMID:18682385). Untested.
• Decisions: all accepted; root catalytic activity -> MODIFY GO:0003852; acyltransferase parent kept non-core.