waaF (PA5012, G3XD35, heptosyltransferase II) review notes

No deep-research provider was available. Sources: UniProt record (TrEMBL; its reaction and family notes
are ARBA-only, so they are not used as evidence), cached PMID:9171387 (abstract only; no full-text XML
obtainable for PMC179135). PubMed MCP searches on 2026-11-05 returned no hits (search service failing).

Function

Cloned by complementing a Salmonella waaF mutant with a PAO1 library
PMID:9171387.
49% identity to Salmonella WaaF; 38-kDa product by maxicell expression
PMID:9171387.
Chromosomal knockouts could not be obtained (merodiploids only).
Family: GT9; NCBIfam TIGR02195 heptsyl_trn_II; InterPro IPR011910 (RfaF). GO:0008713 is defined as
the heptosyltransferase II reaction (heptosyl-Kdo2-lipid A acceptor), which fits WaaF.

PseudoCAP IBA-seed assessment

PTN node	node term	n_seeds	leaf IBAs	seed annotation (term, evidence, PMID)	verdict	rationale	other seeds	IBD stands without this seed?
PTN001575314	GO:0009244 LPS core region biosynthetic process	7	64 (24 taxa)	GO:0009244, IDA, PMID:9171387 (PseudoCAP)	SOUND	Heterologous complementation of the Salmonella waaF mutant; term right for a core heptosyltransferase. Genetic evidence, so IDA is probably the wrong code (SUSPECTED PSEUDOCAP WRONG_EVIDENCE_CODE, abstract only), as for waaC.	E. coli WaaC P24173, WaaF P37692, WaaQ P25742; Salmonella WaaC P26469, WaaF P37421 (all IMP); PAO1 WaaC Q9HUF5	Yes; five enterobacterial IMP seeds. No GO_CENTRAL defect for GO:0009244.

Shared node with waaC (same paper, same verdict). The node-level GO_CENTRAL defect for the GO:0008713
IBD at PTN001575314 (Hep II term over a Hep I/II/III clade) is recorded once, on waaC; for WaaF itself
the GO:0008713 IBA is correct and is accepted here.

Other PseudoCAP row: ISS GO:0071968 lipid A-core heptosyltransferase activity with Salmonella WaaF
P37421: WRONG_TERM, CONFIRMED; the correct term is GO:0008713 (same error as on waaC).

Defects recorded: PSEUDOCAP 2 (WRONG_EVIDENCE_CODE SUSPECTED; WRONG_TERM CONFIRMED); GO_CENTRAL 0 here
(1 node-level defect on waaC).