Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
Muscle-type creatine kinase interacts with central domains of the M-band proteins myomesin and M-protein.
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MM-CK binds the sarcomeric M-band in an isoform-specific manner and acts as an intramyofibrillar ATP-regenerating system for the nearby actin-activated myosin ATPase.
"MM-CK interacts in an isoform-specific manner with the M-band of sarcomeric muscle, where it serves as an efficient intramyofibrillar ATP-regenerating system for the actin-activated myosin ATPase located nearby on both sides of the M-band."
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MM-CK binds central domains of myomesin (My7-8) and of the related M-protein (domains six to eight), identifying its M-band binding partners.
"A yeast two-hybrid screen led to the identification of MM-CK as a binding partner of a central portion of myomesin (My7-8). An interaction was observed with domains six to eight of the closely related M-protein"
Next-generation sequencing to generate interactome datasets.
A proteome-scale map of the human interactome network.
Architecture of the human interactome defines protein communities and disease networks.
A reference map of the human binary protein interactome.
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Isolation and sequence analysis of a full-length cDNA for human M creatine kinase.
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Reports the full-length cDNA and coding sequence for human M-type creatine kinase, with strong cross-species conservation of the enzyme.
"A full length cDNA for human M creatine kinase has been isolated and sequenced."
creatine + ATP => phosphocreatine + ADP [CKB,CKM]