MLA10 (barley, Q6WWJ4) research notes

Identity

• UniProt Q6WWJ4 (TrEMBL, unreviewed), EMBL AY266445 / AAQ55541, 951 aa, CC (Rx_N) - NB-ARC - WHD - LRR.
• Bai et al. 2012 used AY266445 as Mla10 PMID:22685408, so this accession is the MLA10 studied there.
• Saur et al. 2019 used Mla10 GU245946, listed as "Different from NCBI:AY266445.1" PMID:30777147; allele-level identity with Q6WWJ4 is therefore not exact for the AVRA10 recognition data, but both are MLA10.

Effector recognition

• AVRa10 (CSEP0141 allele) isolated; recognised by MLA10 PMID:30777147
• Association: PMID:30777147; yeast: PMID:30777147. No purified-protein binding or MLA10-AVRA10 structure.
• LRR determines specificity PMID:33534797

Localisation and signalling

• Nucleocytoplasmic; nuclear pool needed and sufficient for resistance PMID:22685408
• Cytoplasmic pool drives cell death; resistance separable from death PMID:22685408
• Contrast: wheat homologues Sr33/Sr50 act in the cytosol for both death and resistance PMID:27555587. Open question.
• WRKY/MYB6: PMID:17185563; PMID:23532068
• CC homodimer = minimal cell-death module PMID:21402358
• NB-ARC switch: P-loop K207R inactive; MHD D502V autoactive PMID:22685408. SGT1 required for cell death in N. benthamiana.
• miR9863 targets MLA1 but not MLA10 PMID:25502438 (background).

Curation decisions (6 GOA rows, all IEA)

• GO:0002758 innate immune response-activating signaling pathway: ACCEPT (receptor initiates pathway).
• GO:0006952 defense response: MODIFY -> GO:0050832 defense response to fungus.
• GO:0098542 defense response to other organism (TreeGrafter): MODIFY -> GO:0050832.
• GO:0009626 plant-type HR: KEEP_AS_NON_CORE (real output but separable from resistance).
• GO:0042742 defense response to bacterium (ARBA): REMOVE (no bacterial recognition for any MLA).
• GO:0043531 ADP binding: KEEP_AS_NON_CORE (NB-ARC switch; not directly measured).
• NEW: GO:0140376 innate immune receptor activity (comparators I2, RGA5, RPP1, RPP4, RPP13 carry it with EXP); GO:0005634 nucleus; GO:0005737 cytoplasm.
• Necessity vs participation: MLA10 is the receptor that performs the recognition step and its CC directly engages WRKY1/MYB6, so process terms reflect participation, not just mutant phenotype.
• Deep research (falcon) consulted: file:HORVU/MLA10/MLA10-deep-research-falcon.md.